酶联免疫斑点试验作为检测肾移植受者流感疫苗接种后细胞免疫的灵敏工具。

ELISpot assay as a sensitive tool to detect cellular immunity following influenza vaccination in kidney transplant recipients.

作者信息

Lindemann Monika, Witzke Oliver, Lütkes Peter, Fiedler Melanie, Kreuzfelder Ernst, Philipp Thomas, Roggendorf Michael, Grosse-Wilde Hans

机构信息

Institute of Immunology, University Hospital, Virchowstrasse 171, 45122 Essen, Germany.

出版信息

Clin Immunol. 2006 Sep;120(3):342-8. doi: 10.1016/j.clim.2006.03.002. Epub 2006 Apr 21.

DOI:10.1016/j.clim.2006.03.002

PMID:16631409

Abstract

Enhanced cellular immunity following influenza vaccination has been undetectable in kidney transplant recipients so far. Protection from influenza is dependent on cellular and humoral immunity. Aim of the study was to investigate immune responses before and after vaccination with influenza A and B antigens in 65 kidney transplant recipients. A significant increase in proliferative responses was only observed towards influenza B (P < 0.0001) by lymphocyte transformation test. The enzyme-linked immunospot (ELISpot) assay was more sensitive and detected significant, 3- to 5-fold increases (P < 0.0001) in interferon-gamma secretion using influenza A and B antigens. Furthermore, influenza antibody titers increased significantly (P < 0.0001). At month 1 post-vaccination 85% of patients displayed specific cellular, and 95% or 92% humoral immunity against influenza A and B, respectively. Thus, applying the sensitive ELISpot assay, influenza-specific cellular immunity could be detected for the first time in kidney transplant recipients after vaccination.

摘要

迄今为止，肾移植受者接种流感疫苗后增强的细胞免疫一直无法检测到。预防流感依赖于细胞免疫和体液免疫。本研究的目的是调查65名肾移植受者接种甲型和乙型流感抗原前后的免疫反应。通过淋巴细胞转化试验，仅观察到对乙型流感的增殖反应显著增加（P < 0.0001）。酶联免疫斑点（ELISpot）测定法更敏感，使用甲型和乙型流感抗原检测到干扰素-γ分泌显著增加3至5倍（P < 0.0001）。此外，流感抗体滴度显著增加（P < 0.0001）。接种疫苗后第1个月，85%的患者表现出针对甲型流感的特异性细胞免疫，分别有95%或92%的患者表现出针对乙型流感的体液免疫。因此，应用敏感的ELISpot测定法，首次在肾移植受者接种疫苗后检测到流感特异性细胞免疫。