Metwalli E, Haines D, Becker O, Conzone S, Pantano C G
Department of Material Science and Engineering, Material Research Institute, The Pennsylvania State University, 198 MRI Building, University Park, PA 16802, USA.
J Colloid Interface Sci. 2006 Jun 15;298(2):825-31. doi: 10.1016/j.jcis.2006.03.045. Epub 2006 Mar 27.
The surface properties and structure of mono-, di-, and tri-aminosilane treated glass surfaces were investigated using surface analytical techniques including X-ray photoelectron spectroscopy (XPS), Raman spectroscopy, atomic force microscopy (AFM), and streaming potential. An optimized dip-coating process was demonstrated to produce roughly silane monolayer coverage on the glass surface. The surface charge measurements indicated that aminosilanization converts the glass surface from negative to positive potentials at neutral pH values. Higher positive streaming potential was observed for tri-compared with mono- and di-aminosilane treated glass surfaces. For all aminosilane treated glass samples, the high-resolution N 1s XPS spectra indicated a preferential orientation of the protonated amino-groups towards the glass surface whereas the free amino groups were protruding outward. This study aimed to obtain uniform, reproducibly thin, strongly adhering, internally cross-linked, and high positively charged aminosilane-coated glass surfaces for the attachment of DNA fragments used in microarraying experiments.
使用包括X射线光电子能谱（XPS）、拉曼光谱、原子力显微镜（AFM）和流动电位在内的表面分析技术，研究了经单氨基硅烷、二氨基硅烷和三氨基硅烷处理的玻璃表面的性质和结构。结果表明，通过优化浸涂工艺可在玻璃表面形成大致的硅烷单分子层覆盖。表面电荷测量表明，在中性pH值下，氨基硅烷化作用可使玻璃表面电位由负变正。与经单氨基硅烷和二氨基硅烷处理的玻璃表面相比，经三氨基硅烷处理的玻璃表面观察到更高的正流动电位。对于所有经氨基硅烷处理的玻璃样品，高分辨率N 1s XPS光谱表明，质子化氨基基团优先朝向玻璃表面排列，而游离氨基基团则向外突出。本研究旨在获得均匀、可重复制备的薄、附着力强、内部交联且带高正电荷的氨基硅烷涂层玻璃表面，用于微阵列实验中DNA片段的附着。
