Savouré N, Maudet M, Nicol M, Pélissier M A, Albrecht R, Briand G, Combre A
Biochimie Médicale A, Faculté de Médecine, Rennes, France.
Int J Vitam Nutr Res. 1996;66(4):306-15.
Weanling female SKH1 hairless mice were randomly divided into 4 groups. Each group was fed a particular regimen for 20 weeks: 1) normal basal diet with 5 IU vitamin A/g and 0.45 microgram selenium/g, 2) vitamin A deficient, 3) selenium deficient, and 4) vitamin A plus selenium deficient. Three hours before being sacrificed, half of the animals were subjected to UV A + B irradiation (3 J/cm2). Superoxide dismutases (SOD), catalase, Se glutathione peroxidase activities were determined in dorsal skin homogenates, as well as the concentrations of GSH and of thiobarbituric acid-reactive substances (TBARS), the latter being an index of lipid peroxidation. Ultraviolet light altered the antioxidant defense of mouse skin tissue: GSH level, catalase and Se glutathione peroxidase activities were lowered and SOD was unequally enhanced according to the nutritional status. Vitamin A and Se deficiencies did not perceptibly aggravate the UV-induced oxidative stress, although the former enhanced the decline of catalase expression induced by the irradiation. Probably through an adaptive mechanism, both dietary deficiencies increased the skin reserve of antioxidant GSH and thus appear to modulate the effects caused by reactive oxygen species.
断乳雌性SKH1无毛小鼠被随机分为4组。每组按特定方案喂养20周：1）含5 IU维生素A/克和0.45微克硒/克的正常基础饮食，2）维生素A缺乏，3）硒缺乏，4）维生素A和硒均缺乏。在处死前3小时，一半动物接受紫外线A+B照射（3 J/cm²）。测定背部皮肤匀浆中的超氧化物歧化酶（SOD）、过氧化氢酶、硒谷胱甘肽过氧化物酶活性，以及谷胱甘肽（GSH）和硫代巴比妥酸反应性物质（TBARS，脂质过氧化指标）的浓度。紫外线改变了小鼠皮肤组织的抗氧化防御：根据营养状况，GSH水平、过氧化氢酶和硒谷胱甘肽过氧化物酶活性降低，SOD则不均衡增强。维生素A和硒缺乏虽未明显加重紫外线诱导的氧化应激，尽管前者增强了照射诱导的过氧化氢酶表达下降。可能通过一种适应性机制，两种饮食缺乏均增加了抗氧化剂GSH的皮肤储备，因此似乎可调节活性氧引起的效应。
