从土耳其人类中分离出的对环丙沙星敏感性降低的沙门氏菌菌株对萘啶酸的耐药性

Nalidixic acid resistance in Salmonella strains with decreased susceptibility to ciprofloxacin isolated from humans in Turkey.

作者信息

Ercis Serpil, Erdem Birsel, Hasçelik Gülşen, Gür Deniz

机构信息

Department of Microbiology and Clinical Microbiology, Hacettepe University Faculty of Medicine, Ankara, Turkey.

出版信息

Jpn J Infect Dis. 2006 Apr;59(2):117-9.

PMID:16632912

Abstract

The aim of this study was to evaluate the value of nalidixic acid resistance as an indicator of decreased susceptibility to ciprofloxacin (MIC = 0.125 - 1 mg/L) in Salmonella isolates from humans (n = 620) in Turkey. One isolate was found to be resistant, and the remaining isolates were susceptible to ciprofloxacin with the Clinical and Laboratory Standards Institute breakpoints; however, 75 isolates (12.1%) had decreased susceptibility. Resistance to nalidixic acid was observed in 76 (12.3%) isolates in the disk diffusion test. Seventy-four of these isolates had decreased susceptibility, one was fully resistant, and one isolate was susceptible to ciprofloxacin. One isolate with decreased susceptibility to ciprofloxacin was intermediate to nalidixic acid. Screening with 30-microg nalidixic acid disks had a sensitivity of 98.6% and a specificity of 99.8% for determination of decreased susceptibility to ciprofloxacin.

摘要

本研究的目的是评估在土耳其从人类分离出的沙门氏菌（n = 620）中，萘啶酸耐药性作为对环丙沙星敏感性降低（MIC = 0.125 - 1 mg/L）指标的价值。发现1株分离株耐药，其余分离株根据临床和实验室标准协会的断点对环丙沙星敏感；然而，75株分离株（12.1%）敏感性降低。在纸片扩散试验中，76株（12.3%）分离株对萘啶酸耐药。这些分离株中有74株敏感性降低，1株完全耐药，1株对环丙沙星敏感。1株对环丙沙星敏感性降低的分离株对萘啶酸呈中介性。用30μg萘啶酸纸片进行筛查，对环丙沙星敏感性降低的测定灵敏度为98.6%，特异性为99.8%。