DNA的序列选择性锇氧化：5-甲基胞嘧啶与胞嘧啶之间的有效区分

Sequence-selective osmium oxidation of DNA: efficient distinction between 5-methylcytosine and cytosine.

作者信息

Okamoto Akimitsu, Tainaka Kazuki, Kamei Taku

机构信息

Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Japan.

出版信息

Org Biomol Chem. 2006 May 7;4(9):1638-40. doi: 10.1039/b600401f. Epub 2006 Mar 21.

DOI:10.1039/b600401f

PMID:16633552

Abstract

5-Methylcytosine was distinguished from cytosine using the large difference of their osmium oxidation rates, and this reaction was applied to detection of the cytosine methylation status at a specific site of a long sequence using the formation of a bulge structure by hybridization with a guide DNA.

摘要

利用5-甲基胞嘧啶和胞嘧啶锇氧化速率的巨大差异来区分它们，并且通过与引导DNA杂交形成凸起结构，将该反应应用于检测长序列特定位点的胞嘧啶甲基化状态。