一种带有两个α-L-岩藻糖基残基的木葡聚糖新十一糖亚基。

A new undecasaccharide subunit of xyloglucans with two alpha-L-fucosyl residues.

作者信息

Hisamatsu M, Impallomeni G, York W S, Albersheim P, Darvill A G

机构信息

Complex Carbohydrate Research Center, University of Georgia, Athens 30602.

出版信息

Carbohydr Res. 1991 Apr 2;211(1):117-29. doi: 10.1016/0008-6215(91)84150-d.

DOI:10.1016/0008-6215(91)84150-d

PMID:1663420

Abstract

A new oligosaccharide subunit of xyloglucan was isolated from the beta-(1----4)-endoglucanase digestion products of the xyloglucan in what is referred to as "sycamore extracellular polysaccharides" and found to be an undecasaccharide having two terminal alpha-L-fucopyranosyl residues. The undecasaccharide was structurally characterized by 1H-n.m.r. spectroscopy, fast-atom bombardment mass spectrometry (f.a.b.-m.s.), and glycosyl-residue and glycosyl-linkage composition analyses. The structure of the undecasaccharide was confirmed by digesting it with a hydrolase that releases alpha-D-Xylp-(1----6)-D-Glc from the non-reducing end of xyloglucan oligosaccharides.

摘要

从所谓的“梧桐细胞外多糖”中的木葡聚糖的β-(1→4)-内切葡聚糖酶消化产物中分离出一种新的木葡聚糖寡糖亚基，发现它是一种具有两个末端α-L-呋喃岩藻糖基残基的十一糖。通过1H-核磁共振光谱、快原子轰击质谱（f.a.b.-m.s.）以及糖基残基和糖基连接组成分析对该十一糖进行了结构表征。通过用一种从木葡聚糖寡糖的非还原端释放α-D-Xylp-(1→6)-D-Glc的水解酶消化该十一糖，证实了其结构。