York W S, van Halbeek H, Darvill A G, Albersheim P
Complex Carbohydrate Research Center, University of Georgia, Athens 30602.
Carbohydr Res. 1990 Apr 25;200:9-31. doi: 10.1016/0008-6215(90)84179-x.
A method to determine rapidly the identities and proportions of the oligosaccharide repeating-units in plant cell-wall xyloglucans by 1D 1H-n.m.r. spectroscopy was developed. Six of the most commonly found xyloglucan oligosaccharide subunits (including three subunits that had not been fully characterized previously) were prepared by endo-(1----4)-beta-D-glucanase digestion of xyloglucans from various plant species. The oligosaccharides were reduced to the corresponding oligoglycosyl-alditols, purified, and characterized by glycosyl composition and linkage analysis, 1H-n.m.r. spectroscopy, and f.a.b.-mass spectrometry. Correlations between the 1H-n.m.r. spectra and the structures of the oligoglycosyl-alditols can be used to identify oligoglycosyl-alditols derived from xyloglucans of unknown structure. The identities and relative amounts of the oligosaccharide subunits of xyloglucans isolated from tamarind seed and rapeseed hulls were determined on this basis.
开发了一种通过一维¹H-核磁共振光谱快速测定植物细胞壁木葡聚糖中寡糖重复单元的种类和比例的方法。通过用内切-(1→4)-β-D-葡聚糖酶消化来自各种植物物种的木葡聚糖，制备了六种最常见的木葡聚糖寡糖亚基（包括三种先前未完全表征的亚基）。将寡糖还原为相应的寡糖基糖醇，进行纯化，并通过糖基组成和连接分析、¹H-核磁共振光谱和快原子轰击质谱进行表征。¹H-核磁共振光谱与寡糖基糖醇结构之间的相关性可用于鉴定源自未知结构木葡聚糖的寡糖基糖醇。在此基础上，确定了从罗望子种子和油菜籽壳中分离出的木葡聚糖的寡糖亚基的种类和相对含量。
