Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung 811, Taiwan.
Department of Agronomy, National Chung Hsing University, Taichung 402, Taiwan.
Molecules. 2020 Sep 16;25(18):4256. doi: 10.3390/molecules25184256.
St. John's wort ( L.) is a medicinal plant that alleviates depression and other disorders due to its abundance of active ingredients. Hyperforin, rutin, and melatonin are the main active, and important, ingredients in St. John's wort that alleviate depression. In order to investigate the optimal conditions for accumulating these active ingredients, design of experiments and response surface methodology (RSM) was employed in this study. Two-month-old St John's wort plants were cultivated in growth chambers at varying temperatures, light intensities, and nutrient solution concentrations before analysis by HPLC, for determining differences in hyperforin, rutin, and melatonin content. The results showed that hyperforin and rutin contents were significantly influenced by temperature (18-23 °C) and light intensity (49-147 μmol m s photosynthetic photon flux density (PPFD)), whereas Hoagland's nutrient solution concentration (25-75%) had little effect. The accumulation of melatonin might not be influenced by cultivation conditions. Light intensity and temperature are easily controlled environmental factors in artificial cultivation, both of which are related to secondary metabolite production in the plant. Based on RSM, the optimal conditions for the accumulation of hyperforin and rutin were obtained. The maximum content of hyperforin was 5.6 mg/g, obtained at a temperature of 19 °C, a nutrient solution concentration of 45%, and a light intensity of 49 μmol m s PPFD. The maximum content of rutin was 3.8 mg/g obtained at a temperature of 18 °C, a nutrient solution concentration of 50%, and a light intensity of 147 μmol m s PPFD. This evaluation of suitable conditions for the accumulation of bioactive compounds in St. John's wort can be applied to plant factories on a large scale.
贯叶金丝桃（L.）是一种药用植物，由于其丰富的活性成分，可缓解抑郁和其他疾病。金丝桃素、芦丁和褪黑素是贯叶金丝桃中缓解抑郁的主要活性和重要成分。为了研究积累这些活性成分的最佳条件，本研究采用实验设计和响应面法（RSM）。在通过 HPLC 分析之前，将两个月大的贯叶金丝桃植物在不同温度、光照强度和营养液浓度的生长室中培养，以确定金丝桃素、芦丁和褪黑素含量的差异。结果表明，金丝桃素和芦丁含量受温度（18-23°C）和光照强度（49-147 μmol m s 光合光子通量密度（PPFD））的显著影响，而 Hoagland 营养液浓度（25-75%）影响较小。褪黑素的积累可能不受栽培条件的影响。光照强度和温度是人工栽培中容易控制的环境因素，两者都与植物次生代谢产物的产生有关。基于 RSM，获得了金丝桃素和芦丁积累的最佳条件。金丝桃素含量最高为 5.6 mg/g，在温度为 19°C、营养液浓度为 45%、光照强度为 49 μmol m s PPFD 时获得。芦丁含量最高为 3.8 mg/g，在温度为 18°C、营养液浓度为 50%、光照强度为 147 μmol m s PPFD 时获得。本研究评估了贯叶金丝桃中生物活性化合物积累的适宜条件，可应用于大规模植物工厂。
