Wajs A, Pranovich A, Reunanen M, Willför S, Holmbom B
Technical University of Lodz, Faculty of Biotechnology and Food Sciences, Institute of General Food Chemistry, Stefanowskiego 4/10, 90-924 Lodz, Poland.
Phytochem Anal. 2006 Mar-Apr;17(2):91-101. doi: 10.1002/pca.891.
Solid-phase microextraction (SPME), hydrodistillation and dynamic headspace combined with GC and GC-MS were applied and compared for the analysis of volatile organic compounds (VOCs) from coniferous wood. The SPME conditions (type of fibre, size of wood sample, temperature and exposure time) were optimised, and more than 100 VOCs and semi-volatile compounds extracted and identified from the sapwood and heartwood of Norway spruce (Picea abies). The total number of mono- and sesquiterpenes eluted and identified was similar for the SPME and hydrodistillation methods, but more semi-volatile compounds were released by hydrodistillation. By applying dynamic headspace at room temperature, it was possible to analyse only the most volatile compounds. The qualitative composition of VOCs was similar in spruce sapwood and heartwood, although Z-beta-ocimene occurred only in sapwood while fenchol was present only in heartwood. SPME sampling coupled with GC, applied here to the analysis of VOCs released from stemwood of firs for the first time, is a convenient, sensitive, fast, solvent-free and simple method for the determination of wood volatiles. The technique requires much smaller sample amounts compared with hydrodistillation, and the total amount of VOCs extracted and identified is higher than that obtained by hydrodistillation or dynamic headspace. The relative ratios of the main mono- and sesquiterpenes and -terpenoids were similar using the SPME-GC and hydrodistillation methods.
采用固相微萃取（SPME）、水蒸馏以及动态顶空结合气相色谱（GC）和气相色谱 - 质谱联用（GC-MS）技术，对针叶树木材中的挥发性有机化合物（VOCs）进行分析并比较。优化了SPME条件（纤维类型、木材样品尺寸、温度和暴露时间），从挪威云杉（Picea abies）的边材和心材中提取并鉴定出100多种VOCs和半挥发性化合物。SPME法和水蒸馏法洗脱并鉴定出的单萜和倍半萜总数相似，但水蒸馏法释放出的半挥发性化合物更多。通过在室温下应用动态顶空，仅能分析挥发性最强的化合物。云杉边材和心材中VOCs的定性组成相似，尽管Z-β-罗勒烯仅存在于边材中，而小茴香醇仅存在于心材中。本文首次将SPME采样与GC联用，用于分析冷杉茎木释放的VOCs，这是一种简便、灵敏、快速、无溶剂且简单的测定木材挥发物的方法。与水蒸馏法相比，该技术所需样品量少得多，提取和鉴定出的VOCs总量高于水蒸馏法或动态顶空法。使用SPME-GC法和水蒸馏法时，主要单萜、倍半萜和萜类化合物的相对比例相似。
