Niwa M, Kawaguchi T, Yamashita K, Maeda T, Kurihara M, Kataoka Y, Ozaki M
Department of Pharmacology 2, Nagasaki University School of Medicine, Japan.
Clin Exp Hypertens A. 1991;13(5):799-806. doi: 10.3109/10641969109042083.
The quantitative receptor autoradiographic method we used revealed that specific 125I-endothelin-1 (125I-ET-1) binding sites are highly concentrated in the choroid plexus (ChP), subfornical organ (SFO), lacunosum molecular layer of the hippocampus (LMol) and granular layer of the cerebellum (GC) of the rat brain. High densities of the binding sites were also observed in the laminae I-III and intermediolateral nucleus of the porcine spinal cord. 125I-ET-1 bound to the rat ChP and LMol with a high-affinity and to the SFO and GC with a low-affinity. The possibility that ET-1 acts as a neuropeptide within the central nervous system by interacting with specific receptors would have to be considered.
我们所采用的定量受体放射自显影法显示，特异性¹²⁵I-内皮素-1（¹²⁵I-ET-1）结合位点在大鼠脑脉络丛（ChP）、穹窿下器官（SFO）、海马分子层（LMol）和小脑颗粒层（GC）中高度集中。在猪脊髓的I-III层和中间外侧核中也观察到结合位点的高密度分布。¹²⁵I-ET-1以高亲和力与大鼠ChP和LMol结合，以低亲和力与SFO和GC结合。必须考虑ET-1通过与特异性受体相互作用在中枢神经系统内作为神经肽发挥作用的可能性。
