来自肝癌患者腹水的汤姆森 - 弗里德赖希（T）活性糖蛋白、血型N抗原前体糖蛋白以及具有T活性的N抗原前体糖蛋白。

Thomsen-Friedenreich (T)-active glycoproteins, blood group N antigen precursor glycoproteins and N antigen precursor glycoproteins with T activity from ascitic fluids of liver cancer patients.

Otsuka K, Nishida S, Yuasa H, Hara A, Shirai T, Ohkuma S

Department of Biochemistry, Tokyo College of Pharmacy, Japan.

Int J Biochem. 1991;23(9):827-37. doi: 10.1016/0020-711x(91)90067-w.

Abstract

Five perchloric acid-soluble fractions (PASFs) obtained from ascitic fluids of three patients with primary hepatocellular carcinoma (HCC), a patient with liver metastatic carcinoma (LUC) from ureteral carcinoma and human normal serum (NS) were subjected to DEAE-cellulose column chromatography to separate seven glycoprotein fractions, respectively. 2. In this chromatography, two HCC-PASFs gave a Thomsen-Friedenreich (T)-active glycoprotein, respectively. 3. Other HCC-PASF gave a T-active glycoprotein, two blood group N antigen precursor glycoproteins and an N antigen precursor glycoprotein with T activity. 4. LUC-PASF gave two T-active glycoproteins and an N antigen precursor glycoprotein with T activity. 5. NS-PASF did not give these serologically active glycoproteins.

摘要

从三名原发性肝细胞癌（HCC）患者的腹水、一名输尿管癌肝转移癌（LUC）患者的腹水以及人正常血清（NS）中获得的五个高氯酸可溶级分（PASFs）分别进行DEAE - 纤维素柱色谱分离，以分离出七个糖蛋白级分。2. 在该色谱中，两个HCC - PASFs分别产生了一种具有Thomsen - Friedenreich（T）活性的糖蛋白。3. 其他HCC - PASF产生了一种T活性糖蛋白、两种血型N抗原前体糖蛋白和一种具有T活性的N抗原前体糖蛋白。4. LUC - PASF产生了两种T活性糖蛋白和一种具有T活性的N抗原前体糖蛋白。5. NS - PASF未产生这些具有血清学活性的糖蛋白。