Kurihara M, Shimauchi S, Ohyama K, Yamakawa T, Watanabe H, Hirakawa S, Ohkuma S
Isehara Research Laboratory, Kanto Chemical Co., Inc., Kanagawa, Japan.
Yakugaku Zasshi. 1990 Oct;110(10):783-7. doi: 10.1248/yakushi1947.110.10_783.
One mol perchloric acid-soluble fraction (PASF) obtained from ascitic fluid of a patient (blood group B) with metastatic omentum tumor from ovarian cancer was a glycoprotein fraction containing 35.4% carbohydrate and exhibited A, B and Thomsen-Friedenreich (T) activities. This fraction was separated into three fractions, Frs. 1-3, by a gel filtration with Bio-Gel A-1.5 m column. Among these fractions, Fr. 1 which was obtained in a 5.5% yield to PASF, was a glycoprotein fraction with a high molecular weight, 23.3% carbohydrate and A, B and T activities. Other minor fraction, Fr. 2, and the major fraction, Fr. 3, contained 43.2% and 43.9% carbohydrate, but did not show A, B and T activities, respectively.
从一名患有卵巢癌转移性网膜肿瘤的患者（B血型）腹水中获得的每摩尔高氯酸可溶性组分（PASF）是一种糖蛋白组分，含35.4%的碳水化合物，并表现出A、B和汤姆森 - 弗里德赖希（T）活性。该组分通过使用Bio - Gel A - 1.5m柱进行凝胶过滤分离为三个组分，即组分1 - 3。在这些组分中，以相对于PASF 5.5%的产率获得的组分1是一种高分子量糖蛋白组分，含23.3%的碳水化合物以及A、B和T活性。其他次要组分，即组分2，以及主要组分，即组分3，分别含有43.2%和43.9%的碳水化合物，但均未表现出A、B和T活性。
