通过限制性内切酶分析证明不存在克氏锥虫动基体DNA甲基化

Evidence of absence of Trypanosoma cruzi kinetoplast DNA methylation by restriction endonuclease analysis.

作者信息

Muñoz S, Venegas J, Solari A

机构信息

Departamento de Bioquimica, Facultad de Medicina, Universidad de Chile, Santiago.

出版信息

Int J Parasitol. 1991 Nov;21(7):863-6. doi: 10.1016/0020-7519(91)90156-2.

DOI:10.1016/0020-7519(91)90156-2

PMID:1663492

Abstract

Eight kinetoplast DNA samples from very different T. cruzi zymodemes were digested with the isoschizomer group of enzymes (MspI-HpaII) and (MboI-Sau 3AI), able to detect DNA methylation on cytidine and adenine for the CCGG and GATC sequences, respectively. Restriction digestion analysis of each kDNA with both isoschizomer groups of enzymes did not display a different profile suggesting that maxicircles and minicircles on this trypanosomatid are not methylated.

摘要

从非常不同的克氏锥虫酶解模式中获取的八个动质体DNA样本，用同裂酶组（MspI-HpaII）和（MboI-Sau 3AI）进行消化，这两组酶分别能够检测CCGG和GATC序列中胞嘧啶和腺嘌呤上的DNA甲基化。用这两组同裂酶对每个动质体DNA进行限制性消化分析，未显示出不同的图谱，这表明这种锥虫的大环和小环没有甲基化。