Schmidt M, Hourfar M K, Wahl A, Nicol S-B, Montag T, Roth W K, Seifried E
Institute of Transfusion Medicine and Immunohematology, German Red Cross, Johann Wolfgang Goethe University, Frankfurt, Germany.
Vox Sang. 2006 May;90(4):276-8. doi: 10.1111/j.1423-0410.2006.00763.x.
The optimized scansystem could detect contaminated platelet products within 24 h. However, the system's sensitivity was reduced by a high fluorescence background even in sterile samples, which led to the necessity of a well-trained staff for confirmation of microscope results.
A new protocol of the optimized scansystem with the addition of a fluorescence quencher was evaluated. Pool platelet concentrates contaminated with five transfusion-relevant bacterial strains were tested in a blind study.
In conjunction with new analysis software, the new quenching dye was able to reduce significantly unspecific background fluorescence. Sensitivity was best for Bacillus cereus and Escherichia coli (3 CFU/ml).
The application of a fluorescence quencher enables automated discrimination of positive and negative test results in 60% of all analysed samples.
优化后的扫描系统能够在24小时内检测出受污染的血小板制品。然而，即使在无菌样本中，高荧光背景也会降低该系统的灵敏度，这就需要训练有素的工作人员来确认显微镜检查结果。
对添加了荧光猝灭剂的优化扫描系统新方案进行了评估。在一项盲法研究中，对被五种与输血相关的细菌菌株污染的混合血小板浓缩物进行了检测。
结合新的分析软件，新型猝灭染料能够显著降低非特异性背景荧光。对蜡样芽孢杆菌和大肠杆菌的灵敏度最佳（3 CFU/ml）。
荧光猝灭剂的应用能够在60%的所有分析样本中实现阳性和阴性检测结果的自动判别。
