Kimura Shingo, Yamauchi Hiroshi, Hibino Yuri, Iwamoto Mieko, Sera Koichiro, Ogino Keiki
Department of Environmental and Preventive Medicine, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan.
Basic Clin Pharmacol Toxicol. 2006 May;98(5):496-502. doi: 10.1111/j.1742-7843.2006.pto_217.x.
The urinary concentration of 8-hydroxydeoxyguanine (8-OHdG), which is a biomarker of oxidative DNA damage, was measured in 248 healthy Japanese, and its correlations with life style, urinary metal elements, serum antioxidants, and other plasma or serum factors were investigated. The mean urinary concentration of 8-OHdG was 15.2+/-5.71 ng/mg creatinine. Mean urinary 8-OHdG was not significantly different in terms of age (<45, >or=45), gender, smoking (no, <20, >or=20), and alcohol consumption (no, occasionally, sometimes and usually). Moreover, multiple regression analysis showed a significant association between urinary 8-OHdG and urinary arsenic (As) or chromium (Cr), and a tendency for association between the former and aluminum (Al) and nickel (Ni). Age, gender and plasma or serum factors including antioxidants, lipid peroxide, HbA1c, BUN, and iron did not show such an association. The present study suggests that natural exposure to toxic metal elements such as As, Cr, and Ni may influence oxidative DNA damage in healthy people under usual environmental management. Therefore, the measurement of urinary metals such as As, Ni and Cr is prerequisite for the study of the relationship between urinary 8-OHdG and other variable factors.
在248名健康日本人中测量了作为氧化DNA损伤生物标志物的8-羟基脱氧鸟苷（8-OHdG）的尿浓度，并研究了其与生活方式、尿金属元素、血清抗氧化剂以及其他血浆或血清因子的相关性。8-OHdG的平均尿浓度为15.2±5.71 ng/mg肌酐。平均尿8-OHdG在年龄（<45岁、≥45岁）、性别、吸烟情况（不吸烟、<20支、≥20支）和饮酒情况（不饮酒、偶尔饮酒、有时饮酒和经常饮酒）方面无显著差异。此外，多元回归分析显示尿8-OHdG与尿砷（As）或铬（Cr）之间存在显著关联，且前者与铝（Al）和镍（Ni）之间有相关趋势。年龄、性别以及包括抗氧化剂、脂质过氧化物、糖化血红蛋白、尿素氮和铁在内的血浆或血清因子未显示出此类关联。本研究表明，在通常的环境管理下，自然接触砷、铬和镍等有毒金属元素可能会影响健康人的氧化DNA损伤。因此，测量尿中的砷、镍和铬等金属是研究尿8-OHdG与其他可变因素之间关系的前提条件。
