Environmental Health Science Department, College of Public Health, University of Georgia, Athens, GA, USA.
Environ Int. 2013 Oct;60:112-22. doi: 10.1016/j.envint.2013.08.013. Epub 2013 Sep 14.
Nearly half of the world's population is exposed to household air pollution (HAP) due to long hours spent in close proximity to unvented cooking fires. The effect of woodsmoke exposure on oxidative stress was examined by investigating the association between woodsmoke exposure and biomarkers of DNA oxidation (8-hydroxy-2'-deoxyguanosine [8-OHdG]) and lipid peroxidation (8-isoprostane) among control and intervention stove users. HAP exposure assessment was conducted within the framework of a community-randomized controlled trial of 51 communities in San Marcos Province, Cajamarca Region, Peru. The first morning urine voids after 48h HAP exposure assessment from a subset of 45 control and 39 intervention stove users were analyzed for 8-OHdG and 8-isoprostane. General linear models and correlation analyses were performed. Urinary oxidative stress biomarkers ranged from 11.2 to 2270.0μg/g creatinine (median: 132.6μg/g creatinine) for 8-OHdG and from 0.1 to 4.5μg/g creatinine (median: 0.8μg/g creatinine) for 8-isoprostane among all study subjects (n=84). After controlling for the effects of traffic in the community and eating food exposed to fire among all subjects, cooking time was weakly, but positively associated with urinary 8-OHdG (r=0.29, p=0.01, n=80). Subjects' real-time personal CO exposures were negatively associated with 8-OHdG, particularly the maximum 30-second CO exposure during the sampling period (r=-0.32, p=0.001, n=73). 48h time integrated personal PM2.5 was negatively, but marginally associated with urinary 8-isoprostane (r=-0.21, p=0.09, n=69) after controlling for the effect of distance of homes to the road. Urinary 8-isoprostane levels reported in the available literature are comparable to results found in the current study. However there were relatively high levels of urinary 8-OHdG compared to data in the available literature for 8-OHdG excretion. Results suggest a sustained systemic oxidative stress among these Peruvian women chronically exposed to wood smoke.
由于长时间处于通风不良的烹饪火附近，全球近一半的人口暴露于室内空气污染（HAP）之下。本研究通过调查木烟暴露与控制组和干预组使用火炉的用户的 DNA 氧化（8-羟基-2'-脱氧鸟苷[8-OHdG]）和脂质过氧化（8-异前列腺素）生物标志物之间的关联，来检验木烟暴露对氧化应激的影响。在秘鲁 Cajamarca 地区 San Marcos 省的一个社区随机对照试验框架内进行了 HAP 暴露评估，该试验涉及 51 个社区。在对 45 名对照组和 39 名干预组使用火炉的用户进行 48 小时 HAP 暴露评估后的第一个早晨，对他们的尿液进行了 8-OHdG 和 8-异前列腺素的分析。进行了一般线性模型和相关性分析。所有研究对象（n=84）的尿氧化应激生物标志物范围为 8-OHdG 为 11.2 至 2270.0μg/g 肌酐（中位数：132.6μg/g 肌酐），8-异前列腺素为 0.1 至 4.5μg/g 肌酐（中位数：0.8μg/g 肌酐）。在所有受试者中，在控制社区交通和食用暴露于火的食物的影响后，烹饪时间与尿 8-OHdG 呈弱正相关（r=0.29，p=0.01，n=80）。受试者实时个人 CO 暴露与 8-OHdG 呈负相关，尤其是采样期间最大的 30 秒 CO 暴露（r=-0.32，p=0.001，n=73）。在控制家庭与道路的距离的影响后，48 小时个人 PM2.5 时间积分与尿 8-异前列腺素呈负相关，但仅为边缘相关（r=-0.21，p=0.09，n=69）。与当前研究结果相比，现有文献中报道的尿 8-异前列腺素水平相当。然而，与现有文献中 8-OHdG 排泄的数据相比，尿 8-OHdG 的水平相对较高。研究结果表明，这些秘鲁妇女长期暴露于木烟中会持续发生系统性氧化应激。
