利用PCR技术对稻瘟病菌遗传多样性进行研究及伊朗马赞德兰省交配型等位基因分布测定

Study on genetic diversity of Magnaporthe grisea using PCR and determination of the mating type alleles distribution in Mazandaran province, Iran.

作者信息

Hemmati R, Javan-Nikkhah M, Okovvat S M, Ghazanfari K

机构信息

Department of Plant Protection, College of Agriculture, University of Tehran, Karaj, Iran.

出版信息

Commun Agric Appl Biol Sci. 2005;70(3):311-3.

PMID:16637192

Abstract

The population structure of Magnaporthe grisea, the causal agent of the rice blast, was analyzed in Mazandaran province, using DNA fingerprinting based on RAPD-PCR by means of three primers including "I", "D" and "H". Total DNA of 47 isolates was extracted and amplified according to a specific PCR program. As a result, variable length fragments were generated. Each isolate was subjected to DNA fingerprinting and clonal lineages were determined. Phenetic analysis differentiated three distinct fingerprint lineages. In order to study on fertility status and distribution of the mating type idiomorphs (alleles), 72 monoconidial isolates from Mazandaran province were paired with four standard fertile hermaphrodite isolates. The mating type of 36 isolates was determined as Mat 1-1. The others (36 isolates) did not form any perithecia in pairing with standard isolates

摘要

利用基于随机扩增多态性DNA聚合酶链反应（RAPD-PCR）的DNA指纹技术，借助包括“I”“D”和“H”在内的三种引物，对马赞德兰省稻瘟病病原菌稻瘟病菌的群体结构进行了分析。提取了47个分离株的总DNA，并根据特定的PCR程序进行扩增。结果产生了可变长度的片段。对每个分离株进行DNA指纹分析并确定克隆谱系。聚类分析区分出三个不同的指纹谱系。为了研究交配型特异形态（等位基因）的育性状况和分布，将来自马赞德兰省的72个单孢分离株与四个标准可育雌雄同体分离株进行配对。36个分离株的交配型被确定为Mat 1-1。其他分离株（36个）与标准分离株配对时未形成任何子囊壳。