Taha A S, Fraser W D, Kelly R W, Gemmell C G, Lee F D, Russell R I
Department of Gastroenterology, Royal Infirmary, Glasgow, UK.
Aliment Pharmacol Ther. 1991 Aug;5(4):379-89. doi: 10.1111/j.1365-2036.1991.tb00041.x.
The effect of Helicobacter pylori protein on cAMP and prostaglandin (PGE2) production was studied in incubates of human gastric fundic mucosa. At 24 hours, specimens incubated in the control fluid had a median cAMP value of 81 pmol/mg protein, compared to 28 pmol/mg (P less than 0.05) when incubated in H. pylori protein, 155 pmol/kg (P less than 0.006) in histamine, and 23 pmol/kg (P less than 0.05) in histamine plus H. pylori protein. A similar trend was observed at 48 hours. Although H. pylori protein had no direct effect on mucosal PGE2, it intensified the inhibitory effect of indomethacin and prevented the stimulatory effect of histamine on both PGE2 and cAMP production. Given the role of cAMP in various physiological responses, these results suggest that H. pylori protein might alter those functional aspects of the human gastric mucosa which rely on cAMP as a second messenger. Assuming that PGE2 is involved in mediating such effect, its role would appear to be either partial or indirect.
在人胃底黏膜培养物中研究了幽门螺杆菌蛋白对环磷酸腺苷（cAMP）和前列腺素（PGE2）产生的影响。24小时时，在对照液中培养的标本的cAMP中位数为81 pmol/mg蛋白，而在幽门螺杆菌蛋白中培养时为28 pmol/mg（P<0.05），在组胺中为155 pmol/kg（P<0.006），在组胺加幽门螺杆菌蛋白中为23 pmol/kg（P<0.05）。48小时时观察到类似趋势。虽然幽门螺杆菌蛋白对黏膜PGE2没有直接影响，但它增强了吲哚美辛的抑制作用，并阻止了组胺对PGE2和cAMP产生的刺激作用。鉴于cAMP在各种生理反应中的作用，这些结果表明幽门螺杆菌蛋白可能会改变人胃黏膜那些依赖cAMP作为第二信使的功能方面。假设PGE2参与介导这种作用，其作用似乎是部分的或间接的。
