Hosni R, Chabannes B, Pacheco Y, Moliere P, Grosclaude M, Perrin Fayolle M, Lagarde M
Hôpital Sainte-Eugénie, Saint Genis Laval, France.
Eur J Clin Invest. 1991 Dec;21(6):631-7. doi: 10.1111/j.1365-2362.1991.tb01420.x.
Leukotriene B4 (LTB4) levels were measured in peripheral blood neutrophils from allergic and healthy donors after stimulation by calcium ionophore A 23187. This level was higher in neutrophils from allergic subjects than in neutrophils from healthy subjects in the presence as well as in the absence of exogenous arachidonic acid. Platelets from allergics increased LTB4 levels from neutrophils from allergics but not levels in those from healthy donors. Moreover, platelets from healthy subjects reduced LTB4 in neutrophils from both groups. These results suggest that biochemical differences exist in neutrophils and platelets from allergics which contribute to changes in arachidonic acid metabolism via the 5-lipoxygenase pathway. In addition, they support the concept that platelets may play an important role in the regulation of neutrophil LTB4 levels, possibly by affecting the 5-lipoxygenase activity during the course of allergic inflammatory reactions.
在钙离子载体A 23187刺激后，测量了过敏供体和健康供体外周血中性粒细胞中的白三烯B4（LTB4）水平。在有或没有外源性花生四烯酸存在的情况下，过敏受试者中性粒细胞中的该水平均高于健康受试者中性粒细胞中的水平。过敏者的血小板会增加过敏者中性粒细胞中的LTB4水平，但不会增加健康供体中性粒细胞中的LTB4水平。此外，健康受试者的血小板会降低两组中性粒细胞中的LTB4水平。这些结果表明，过敏者的中性粒细胞和血小板存在生化差异，这些差异通过5-脂氧合酶途径导致花生四烯酸代谢发生变化。此外，它们支持这样一种观点，即血小板可能在中性粒细胞LTB4水平的调节中起重要作用，可能是通过在过敏性炎症反应过程中影响5-脂氧合酶活性来实现的。
