孕早期应用前列腺素E2后人子宫颈中的胶原酶活性

Collagenase activity in the human cervix uteri after prostaglandin E2 application during the first trimester.

作者信息

Osmers R, Rath W, Adelmann-Grill B C, Fittkow C, Szeverényi M, Kuhn W

机构信息

Department of Obstetrics and Gynaecology, University of Göttingen, F.R.G.

出版信息

Eur J Obstet Gynecol Reprod Biol. 1991 Nov 3;42(1):29-32. doi: 10.1016/0028-2243(91)90155-e.

DOI:10.1016/0028-2243(91)90155-e

PMID:1663873

Abstract

Cervix biopsies were obtained during the first trimester from plurigravidae and primigravidae at various times after intracervical application of prostaglandin E2. The tissues were extracted with Ca(2+)-containing buffer, and collagenase activity was determined in these extracts using a solid phase assay in which triple helical 125I-labelled collagen was cleaved. Collagenase was detected in all samples but elevated activity was present only during a short temporal window after prostaglandin application. Maximal activity was observed 1 and 2 h after application of prostaglandin in multigravidae and 4 h in primigravidae. These data can explain why collagenase activity in cervical tissues after prostaglandin application had previously not been found. They indicate somewhat different mechanisms of cervical ripening in primigravide compared to multigravidae.

摘要

在妊娠早期，对经宫颈应用前列腺素E2后不同时间的经产妇和初产妇进行宫颈活检。组织用含Ca(2+)的缓冲液提取，使用固相分析法测定这些提取物中的胶原酶活性，该方法中三螺旋125I标记的胶原被裂解。在所有样本中均检测到胶原酶，但仅在应用前列腺素后的短时间窗口内活性升高。经产妇在应用前列腺素后1小时和2小时观察到最大活性，初产妇在4小时观察到最大活性。这些数据可以解释为什么以前未发现应用前列腺素后宫颈组织中的胶原酶活性。它们表明初产妇与经产妇相比，宫颈成熟的机制略有不同。