Romalde J L, Iteman I, Carniel E
Unité de Bactériologie Moléculaire et Medicale, Institut Pasteur, Paris, France.
FEMS Microbiol Lett. 1991 Nov 15;68(2):217-25. doi: 10.1016/0378-1097(91)90130-3.
Field inversion gel electrophoresis (FIGE) and contour-clamped homogeneous field (CHEF) electrophoresis were used to analyse the chromosome of Yersinia ruckeri. The 8 base-pair recognition endonucleases, NotI and SfiI, generated less than 47 DNA fragments whose size and distribution were appropriate for pulsed field separation. Each isolate displayed a characteristic restriction pattern, with about 20% of bands in common. Depending on the strain used, the estimated genome size for this bacterial fish pathogen ranged from 4460 to 4770 kilobase pairs.
