Moraleda G, Bartolomé J, Molina J, Castillo I, Carreño V
Department of Gastroenterology, Fundación Jiménez Díaz, Madrid, Spain.
Liver. 1991 Dec;11(6):352-7. doi: 10.1111/j.1600-0676.1991.tb00542.x.
Hepatitis B virus DNA (HBV-DNA) in serum was studied in 67 anti-HBe patients using dot-blot hybridization, a modified technique and polymerase chain reaction (PCR). All patients had abnormal aminotransferase (ALT) levels. Serum HBV-DNA was detected in 14/67 cases by dot-blot and in 39/67 (DNA probe) and 45/67 (RNA probe) using the modified technique. The RNA probe was more sensitive than the DNA probe when they were compared, using serial dilutions of HBV-DNA of known concentration (0.1 pg vs 1 pg, respectively). HBV-DNA was found by PCR in 57/67 patients. Ten patients were negative to serum HBV-DNA. The ALT level was significantly higher in patients with serum HBV-DNA by dot-blot as compared to those who had serum HBV-DNA by the modified technique and PCR. With respect to the presence of anti-HCV, 6/67 had anti-HCV confirmed by RIBA test. These 6 patients had serum HBV-DNA. The route of acquisition of HBV infection in anti-HCV positive patients was by parenteral exposure in 67% of the cases and 15% in HCV negative (p less than 0.05). All patients were negative to nonorganic specific autoantibodies. In summary, most of the anti-HBe patients (85%) had viral replication. HCV superinfection plays a minor role in the activity of liver disease.
采用斑点杂交、改良技术和聚合酶链反应（PCR）对67例抗HBe患者血清中的乙型肝炎病毒DNA（HBV-DNA）进行了研究。所有患者的转氨酶（ALT）水平均异常。通过斑点杂交在14/67例患者中检测到血清HBV-DNA，采用改良技术时，在39/67例（DNA探针）和45/67例（RNA探针）中检测到血清HBV-DNA。当使用已知浓度的HBV-DNA系列稀释液（分别为0.1 pg和1 pg）进行比较时，RNA探针比DNA探针更敏感。通过PCR在57/67例患者中发现了HBV-DNA。10例患者血清HBV-DNA呈阴性。与采用改良技术和PCR检测到血清HBV-DNA的患者相比，通过斑点杂交检测到血清HBV-DNA的患者ALT水平显著更高。关于抗HCV的存在，6/67例患者通过重组免疫印迹法（RIBA）检测确诊为抗HCV。这6例患者血清HBV-DNA呈阳性。抗HCV阳性患者感染HBV的途径为经肠外暴露，占67%，而HCV阴性患者为15%（P<0.05）。所有患者非器官特异性自身抗体均为阴性。总之，大多数抗HBe患者（85%）存在病毒复制。HCV重叠感染在肝病活动中起次要作用。
