Nakayama Tatsuya, Ito Emi, Nomura Nakao, Nomura Nobuhiko, Matsumura Masatoshi
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
FEMS Microbiol Lett. 2006 May;258(2):194-9. doi: 10.1111/j.1574-6968.2006.00225.x.
Vibrio harveyi strains isolated from shrimp farms (wild strains) were compared with those from culture collections in terms of minimum inhibitory concentration (MIC) and toxicity. Wild strains had higher MIC values for four antibiotics (kanamycin, carbenicillin, oxytetracycline and ampicillin) and also showed higher toxicity compared with culture collection strains. Vibrio harveyi with the lowest antibacterial resistance was chosen to test if a gradual increase in antibiotic concentration and frequent subculture would enhance its antibiotic resistance. Results showed that V. harveyi was able to develop resistance to oxytetracycline. The MIC value was 250 times higher compared with the MIC before subculturing. Moreover, the V. harveyi strain developed slightly higher toxicity. Therefore, it is possible that there is a relationship between antibiotic resistance and toxicity in V. harveyi.
对从虾养殖场分离出的哈维氏弧菌菌株（野生菌株）与保藏菌株在最低抑菌浓度（MIC）和毒性方面进行了比较。野生菌株对四种抗生素（卡那霉素、羧苄青霉素、土霉素和氨苄青霉素）的MIC值更高，并且与保藏菌株相比毒性也更高。选择抗菌抗性最低的哈维氏弧菌来测试抗生素浓度的逐渐增加和频繁传代是否会增强其抗生素抗性。结果表明，哈维氏弧菌能够对土霉素产生抗性。与传代培养前的MIC相比，MIC值高出250倍。此外，哈维氏弧菌菌株的毒性略有增加。因此，哈维氏弧菌的抗生素抗性与毒性之间可能存在关联。
