Droubogiannis Stavros, Katharios Pantelis
Institute of Marine Biology, Biotechnology & Aquaculture, Hellenic Centre for Marine Research, 71500 Heraklion, Greece.
Department of Biology, School of Sciences and Engineering, University of Crete, 71500 Heraklion, Greece.
Pathogens. 2022 May 30;11(6):630. doi: 10.3390/pathogens11060630.
Due to the emergence of multidrug-resistant bacteria, commonly known as "superbugs", phage therapy for the control of bacterial diseases rose in popularity. In this context, the use of phages for the management of many important bacterial diseases in the aquaculture environment is auspicious. a well-known and serious bacterial pathogen, is responsible for many disease outbreaks in aquaculture, resulting in huge economic and production losses. We isolated and fully characterized a novel bacteriophage, Vibrio phage Virtus, infecting strain VH2. Vibrio phage Virtus can infect a wide spectrum of spp., including strains of , , , , and . It has a latent period of 40 min with an unusually high burst size of 3200 PFU/cell. Vibrio phage Virtus has a double-stranded DNA of 82,960 base pairs with 127 predicted open reading frames (ORFs). No virulence, antibiotic resistance, or integrase-encoding genes were detected. In vivo phage therapy trials in gilthead seabream, , larvae demonstrated that Vibrio phage Virtus was able to significantly improve the survival of larvae for five days at a multiplicity of infection (MOI) of 10, which suggests that it can be an excellent candidate for phage therapy.
由于多重耐药细菌（俗称“超级细菌”）的出现，用于控制细菌疾病的噬菌体疗法越来越受欢迎。在这种情况下，利用噬菌体来管理水产养殖环境中的许多重要细菌疾病前景良好。一种著名且严重的细菌病原体，在水产养殖中引发了许多疾病爆发，导致巨大的经济和生产损失。我们分离并全面表征了一种新型噬菌体——弧菌噬菌体Virtus，它可感染VH2菌株。弧菌噬菌体Virtus能感染多种弧菌属物种，包括 、 、 、 和 的菌株。其潜伏期为40分钟，裂解量异常高，达3200噬菌斑形成单位/细胞。弧菌噬菌体Virtus具有一条82960个碱基对的双链DNA，带有127个预测的开放阅读框（ORF）。未检测到毒力、抗生素抗性或整合酶编码基因。在金头鲷幼体中的体内噬菌体治疗试验表明，弧菌噬菌体Virtus在感染复数（MOI）为10时，能够显著提高幼体五天的存活率，这表明它可能是噬菌体治疗的优秀候选者。
