Lu Jun-ping, Zheng Li-xing, Cai De-pei
Children's Hospital of Fudan University, Shanghai 200032, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2006 Mar;40(2):88-92.
To investigate the role of environmental endocrine disruptors (EEDs) in causing the precocious puberty.
The blood samples were collected from 79 cases of precocious puberty patients and 42 cases of normal children. The concentrations of 4-nonylphenol (4-NP), 1, 1-dichloro-2, 2, bis (p-chlorophenyl) ethylene (p, p'-DDE) and di-2-ethylhexyl phthalate (DEHP) in blood serum samples were measured by using reversed-phase high performance liquid chromatography (HPLC). The volume of uterus and ovary, the bone density, and the content of estradiol (E(2)) in serum were determined at the same time. The contents of EEDs in blood serums of precocious puberty and the indices of the target organs were analyzed by using of correlation and regression.
In normal control group, p, p'-DDE was detected in all the blood samples (14.93 - 40.39 ng/ml), but 4-NP and DEHP were detected in some samples (ND -6.77 ng/ml, ND -17.61 ng/ml). The levels of 4-NP, p, p'-DDE and DEHP in blood serum in precocious puberty group were notably increased than that in control group (P < 0.01). In precocious puberty group, there was a positive correlations between the 4-NP in volume of uterus and the volume of ovary and the density of bone (r = 0.394, 0.286, 0.237, P < 0.01); p, p'-DDE and volume of uterus also showed a the positive correlation (r = 0.306, P < 0.01). The influencing extent of 4-NP was 1.3 times to that of the p, p'-DDE.
The normal children and the children with precocious puberty should be all contaminated by EEDs, and the later be exposured to more EEDs. There might exist a close relationship between EEDs and the precocious puberty, and EEDs should be an important factor in causing the disease. Different kinds of EEDs might have different influencing extents to the target organs.
探讨环境内分泌干扰物（EEDs）在导致性早熟中的作用。
采集79例性早熟患儿和42例正常儿童的血样。采用反相高效液相色谱法（HPLC）测定血清样本中4-壬基酚（4-NP）、1,1-二氯-2,2-双（对氯苯基）乙烯（p,p'-DDE）和邻苯二甲酸二（2-乙基己基）酯（DEHP）的浓度。同时测定子宫和卵巢体积、骨密度以及血清雌二醇（E₂）含量。运用相关性和回归分析性早熟患儿血清中EEDs含量与靶器官指标的关系。
正常对照组所有血样均检测到p,p'-DDE（14.93 - 40.39 ng/ml），部分样本检测到4-NP和DEHP（ND - 6.77 ng/ml，ND - 17.61 ng/ml）。性早熟组血清中4-NP、p,p'-DDE和DEHP水平显著高于对照组（P < 0.01）。性早熟组中，4-NP与子宫体积、卵巢体积和骨密度呈正相关（r = 0.394、0.286、0.237，P < 0.01）；p,p'-DDE与子宫体积也呈正相关（r = 0.306，P < 0.01）。4-NP的影响程度是p,p'-DDE的1.3倍。
正常儿童和性早熟儿童均受到EEDs污染，且性早熟儿童接触的EEDs更多。EEDs与性早熟之间可能存在密切关系，EEDs应是导致该病的重要因素。不同种类的EEDs对靶器官的影响程度可能不同。
