一种用于流感嗜血杆菌β-内酰胺酶基因的多重PCR及一种新型blaTEM启动子变体的描述。
A multiplex PCR for beta-lactamase genes of Haemophilus influenzae and description of a new blaTEM promoter variant.
作者信息
Tristram Stephen G, Nichols Scott
机构信息
School of Human Life Sciences, University of Tasmania Launceston, Tasmania 7250, Australia.
出版信息
J Antimicrob Chemother. 2006 Jul;58(1):183-5. doi: 10.1093/jac/dkl150. Epub 2006 Apr 26.
OBJECTIVES
To establish a multiplex PCR to detect and differentiate the beta-lactamase genes of Haemophilus influenzae.
METHODS
A collection of H. influenzae isolates with a range of different beta-lactamase genes were tested in parallel with individual PCRs and the multiplex assay developed in this study.
RESULTS
The multiplex method was found to be superior to previous methods and was able to correctly detect and differentiate blaROB-1 and the variants of blaTEM present in the strains tested, including a previously unrecognized variant associated with a 54 bp insertion in the promoter region.
CONCLUSIONS
The multiplex PCR will be a useful tool for the surveillance of beta-lactamase genes in H. influenzae.
目的
建立一种多重聚合酶链反应（PCR）以检测和区分流感嗜血杆菌的β-内酰胺酶基因。
方法
对一系列携带不同β-内酰胺酶基因的流感嗜血杆菌分离株，同时采用单个PCR及本研究开发的多重检测方法进行检测。
结果
发现该多重方法优于以往方法，能够正确检测和区分测试菌株中存在的blaROB-1和blaTEM变体，包括一种先前未识别的与启动子区域54 bp插入相关的变体。
结论
该多重PCR将成为监测流感嗜血杆菌β-内酰胺酶基因的有用工具。
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