Rooney J F, Wohlenberg C R, Moss B, Notkins A L
Laboratory of Oral Medicine, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892.
Rev Infect Dis. 1991 Nov-Dec;13 Suppl 11:S898-903. doi: 10.1093/clind/13.supplement_11.s898.
Vaccinia virus recombinants expressing antigens from herpes simplex virus (HSV) have been tested as potential live virus vaccines for prevention of HSV infection. We describe three vaccinia virus/HSV recombinants. The first expresses the HSV-1 glycoprotein D (vaccinia/gD), the second expresses the HSV-1 glycoprotein B (vaccinia/gB), and the third expresses both the HSV-1 glycoprotein D and the influenza A hemagglutinin (vaccinia/HSVgD/influenza). Mice immunized with vaccinia/gD or vaccinia/gB developed antibodies capable of neutralizing HSV in vitro and were protected against both lethal and latent infection with HSV. Protection against HSV challenge persisted for greater than 1 year in mice immunized with vaccinia/gD. The immune response to HSV in mice immunized with vaccinia/gD could be increased by a booster vaccination with vaccinia/gD. However, the immune response to HSV was decreased in animals immunized with a vaccinia recombinant that expressed non-HSV genes before vaccination with vaccinia/gD. In separate experiments, a bivalent vaccinia recombinant, vaccinia/HSVgD/influenza, was constructed and was found to be comparable to the vaccinia/gD single recombinant in immunogenicity and protective efficacy against lethal HSV challenge. We conclude that vaccinia/HSV recombinants can provide protection against HSV infection in mice and that these recombinants may provide an alternative approach in the development of a live virus vaccine against HSV.
表达单纯疱疹病毒（HSV）抗原的痘苗病毒重组体已作为预防HSV感染的潜在活病毒疫苗进行了测试。我们描述了三种痘苗病毒/HSV重组体。第一种表达HSV-1糖蛋白D（痘苗/gD），第二种表达HSV-1糖蛋白B（痘苗/gB），第三种表达HSV-1糖蛋白D和甲型流感血凝素（痘苗/HSVgD/流感）。用痘苗/gD或痘苗/gB免疫的小鼠产生了能够在体外中和HSV的抗体，并对HSV的致死性和潜伏性感染具有抵抗力。在用痘苗/gD免疫的小鼠中，对HSV攻击的抵抗力持续超过1年。用痘苗/gD加强免疫可增强用痘苗/gD免疫的小鼠对HSV的免疫反应。然而，在用表达非HSV基因的痘苗重组体免疫的动物中，在用痘苗/gD免疫之前，对HSV的免疫反应降低。在单独的实验中，构建了一种二价痘苗重组体痘苗/HSVgD/流感，发现其在免疫原性和对致死性HSV攻击的保护效力方面与痘苗/gD单一重组体相当。我们得出结论，痘苗/HSV重组体可以为小鼠提供针对HSV感染的保护，并且这些重组体可能为开发针对HSV的活病毒疫苗提供一种替代方法。
