Blacklaws B A, Krishna S, Minson A C, Nash A A
Department of Pathology, University of Cambridge, United Kingdom.
Virology. 1990 Aug;177(2):727-36. doi: 10.1016/0042-6822(90)90539-4.
Vaccinia virus recombinants expressing glycoproteins B (vgB11), D (VgD52), E (gE/7.5 and gE/4B), G (gG-vac), H (gH-vac), and I (gI-vac) of HSV-1 were used to compare the protective response to these individual glycoproteins in the mouse. Glycoprotein D induced the best neutralizing antibody titers and the most increased rates of HSV clearance from the ear as well as good protection from the establishment of latent HSV infections in the sensory ganglia. Glycoprotein B also induced good neutralizing antibody titers and as great a protection from the establishment of latency as gD although the rate of virus clearance from the ear was not as great as after immunization with gD. Glycoprotein E induced weak neutralizing antibody but gG, gH, and gI did not show a neutralizing antibody response. At higher challenge doses of virus (10(6) PFU HSV-1 in the ear), gE induced a protective response by increasing the rate of virus clearance and reducing the acute infection of ganglia as compared to negative control immunized mice. However there was no protection from the establishment of latent infections after immunization with gE. No protective response was seen to gG, gH, or gl.
使用表达单纯疱疹病毒1型糖蛋白B（vgB11）、D（VgD52）、E（gE/7.5和gE/4B）、G（gG-vac）、H（gH-vac）和I（gI-vac）的痘苗病毒重组体，在小鼠中比较对这些单个糖蛋白的保护性反应。糖蛋白D诱导出最佳的中和抗体滴度以及耳部单纯疱疹病毒清除率的最大增幅，并且对感觉神经节中潜伏性单纯疱疹病毒感染的建立有良好的保护作用。糖蛋白B也诱导出良好的中和抗体滴度，并且在防止潜伏感染建立方面与糖蛋白D具有同样好的保护作用，尽管耳部病毒清除率不如用糖蛋白D免疫后那么高。糖蛋白E诱导出较弱的中和抗体，但糖蛋白G、H和I未显示出中和抗体反应。在较高的病毒攻击剂量（耳部接种10(6) 个空斑形成单位单纯疱疹病毒1型）下，与阴性对照免疫的小鼠相比，糖蛋白E通过提高病毒清除率和减少神经节的急性感染诱导出保护性反应。然而，用糖蛋白E免疫后不能防止潜伏感染的建立。未观察到对糖蛋白G、H或I的保护性反应。
