Weber Friedemann, Wagner Valentina, Rasmussen Simon B, Hartmann Rune, Paludan Søren R
Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Universität Freiburg, D-79008 Freiburg, Germany.
J Virol. 2006 May;80(10):5059-64. doi: 10.1128/JVI.80.10.5059-5064.2006.
Double-stranded RNA (dsRNA) longer than 30 bp is a key activator of the innate immune response against viral infections. It is widely assumed that the generation of dsRNA during genome replication is a trait shared by all viruses. However, to our knowledge, no study exists in which the production of dsRNA by different viruses is systematically investigated. Here, we investigated the presence and localization of dsRNA in cells infected with a range of viruses, employing a dsRNA-specific antibody for immunofluorescence analysis. Our data revealed that, as predicted, significant amounts of dsRNA can be detected for viruses with a genome consisting of positive-strand RNA, dsRNA, or DNA. Surprisingly, however, no dsRNA signals were detected for negative-strand RNA viruses. Thus, dsRNA is indeed a general feature of most virus groups, but negative-strand RNA viruses appear to be an exception to that rule.
长度超过30个碱基对的双链RNA（dsRNA）是针对病毒感染的先天性免疫反应的关键激活因子。人们普遍认为，基因组复制过程中双链RNA的产生是所有病毒共有的特征。然而，据我们所知，尚无研究对不同病毒产生双链RNA的情况进行系统调查。在此，我们利用一种dsRNA特异性抗体进行免疫荧光分析，研究了一系列病毒感染的细胞中dsRNA的存在情况和定位。我们的数据显示，正如预期的那样，对于基因组由正链RNA、双链RNA或DNA组成的病毒，可以检测到大量的dsRNA。然而，令人惊讶的是，对于负链RNA病毒未检测到dsRNA信号。因此，双链RNA确实是大多数病毒群体的一个普遍特征，但负链RNA病毒似乎是该规则的一个例外。
