Krohn Thomas C, Itter Gabi, Fosse Richard, Hansen Axel K
Centre for Applied Laboratory Animal Research, Department of Veterinary Pathobiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
J Am Assoc Lab Anim Sci. 2006 May;45(3):51-3.
The reduction and control of allergens in the animal facility is important for staff working with laboratory animals. This study was designed to evaluate the efficiency of perforated Makrolon curtains in front of racks as a method to reduce the amount of allergen in the animal room. The experimental situation we studied provides some information regarding allergen disposition in animal rooms but is clearly artificial and does not reflect a typical, 'real-world' environment in terms of preventing exposure of workers to allergens. Plastic curtains with holes were placed in front of racks, and a corridor between the racks and a curtain was present. The room was ventilated with air, which was blown into the room through the middle of the corridor, flowing downstream and passing through the holes in the curtain. This set-up resulted in air flow from the corridor through the curtain. Air samples were collected from sites in the corridor and behind the curtain. The samples were analyzed for the allergen Mus m1, and the amount of allergen was calculated. The results show air flow from the aisle through the holes in the curtains and through the racks behind the curtains, and this flow keeps allergen behind the curtains and prevents its spread from the cages into the aisle. The present study shows that the use of curtains in front of the cage racks is an efficient way to prevent spread of allergens from rodent cages to the entire animal room.
对于在动物设施中工作的人员而言，减少和控制动物设施中的过敏原至关重要。本研究旨在评估架前穿孔的聚碳酸酯窗帘作为减少动物房内过敏原量的一种方法的有效性。我们所研究的实验情况提供了一些关于动物房内过敏原分布的信息，但显然是人为设置的，在防止工作人员接触过敏原方面并不能反映典型的“现实世界”环境。在架前放置有孔的塑料窗帘，且在架子和窗帘之间有一条走廊。房间通过空气通风，空气从走廊中间吹入房间，向下流动并穿过窗帘上的孔。这种设置导致空气从走廊穿过窗帘。从走廊和窗帘后面的位置采集空气样本。对样本进行过敏原小鼠主要尿蛋白（Mus m1）分析，并计算过敏原量。结果表明空气从过道通过窗帘上的孔并穿过窗帘后面的架子，这种气流将过敏原留在窗帘后面，防止其从笼子扩散到过道。本研究表明，在笼架前使用窗帘是防止过敏原从啮齿动物笼扩散到整个动物房的有效方法。
