Wachtel S, Demas S, Tiersch T, Pechan P, Shapiro D
Department of Obstetrics and Gynecology, University of Tennesse, Memphis 38105.
Genome. 1991 Aug;34(4):612-7. doi: 10.1139/g91-093.
We studied DNA from the protogynous sex-changing fish Anthias squamipinnis to evaluate the recent observation that male-specific bands are identified after hybridization with Bkm, a probe originating in the W chromosome of the snake Bungarus fasciatus. Sex-specific hybridization would imply modification of DNA structure during the sex-changing process. No sex-specific Bkm fragments were identified in our study, after digestion of DNA from 15 males and 11 adult females, despite the use of 12 different restriction enzymes. However, hybridization with Bkm did produce a distinct fingerprint pattern, similar to the fingerprint patterns described for other species after hybridization with GATA (GACA) type probes. In other experiments, the pDP1007 probe, which identifies the ZFY gene in the male-determining region of the human Y chromosome, generated identical hybridization patterns in DNA from males and females of A. squamipinnis and estimation of DNA mass by flow cytometry revealed identical genome sizes.
我们研究了雌性先熟的性转变鱼类——栉鳍天竺鲷（Anthias squamipinnis）的DNA，以评估最近的一项观察结果，即与Bkm杂交后可鉴定出雄性特异性条带，Bkm是一种源自眼镜蛇（Bungarus fasciatus）W染色体的探针。性别特异性杂交意味着在性转变过程中DNA结构发生了改变。在我们的研究中，尽管使用了12种不同的限制性内切酶，但在对15只雄性和11只成年雌性的DNA进行消化后，未鉴定出性别特异性的Bkm片段。然而，与Bkm杂交确实产生了一种独特的指纹图谱模式，类似于其他物种与GATA（GACA）型探针杂交后所描述的指纹图谱模式。在其他实验中，可识别人类Y染色体雄性决定区域中ZFY基因的pDP1007探针，在栉鳍天竺鲷的雄性和雌性DNA中产生了相同的杂交模式，并且通过流式细胞术对DNA质量的估计显示基因组大小相同。
