Lloyd M A, Fields M J, Thorgaard G H
Program in Genetics and Cell Biology, Washington State University, Pullman 99164-4350.
Genome. 1989 Oct;32(5):865-8. doi: 10.1139/g89-523.
GATA-GACA repetitive sequences first isolated from a female snake (termed BKm sequences) and associated with sex chromosomes in some species were hybridized to DNA from rainbow trout (Salmo gairdneri). Genomic DNA was studied from three groups of rainbow trout: (i) randomly selected males and females from an outbred group, (ii) androgenetic individuals from an inbred strain, and (iii) parents and offspring of an outbred strain. Three restriction enzymes (EcoRI, HaeIII, or HinfI) were used to digest the genomic DNA. The DNA was electrophoresed in agarose gels, transferred to nylon membranes, and the GATA-GACA repetitive sequence probe was hybridized to this DNA. There was no evidence of sex-associated patterns of hybridization with the enzymes used. However, the sequences reveal DNA fingerprint polymorphisms which appear to be inherited in a stable manner.
最初从一条雌性蛇中分离出来（称为BKm序列）并与某些物种的性染色体相关联的GATA - GACA重复序列，与虹鳟（Salmo gairdneri）的DNA进行了杂交。对三组虹鳟的基因组DNA进行了研究：（i）从一个远交群体中随机选择的雄性和雌性，（ii）来自一个近交品系的雄核发育个体，以及（iii）一个远交品系的亲本和后代。使用三种限制性内切酶（EcoRI、HaeIII或HinfI）消化基因组DNA。将DNA在琼脂糖凝胶中进行电泳，转移到尼龙膜上，然后将GATA - GACA重复序列探针与该DNA杂交。没有证据表明所用酶与性别相关的杂交模式。然而，这些序列揭示了似乎以稳定方式遗传的DNA指纹多态性。
