Ventura Roland A, Martin-Subero Jose I, Jones Margaret, McParland Joanna, Gesk Stefan, Mason David Y, Siebert Reiner
LRF Immunodiagnostics Unit, Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, Oxford, United Kingdom.
J Mol Diagn. 2006 May;8(2):141-51. doi: 10.2353/jmoldx.2006.050083.
Over the last decade, fluorescence in situ hybridization (FISH) has become a firmly established technique in the diagnosis and assessment of lymphoid malignancies. However, this technique is not wide-ly used in the routine diagnostic evaluation of paraffin-embedded biopsies, most likely because of a perception that it is technically more demanding. There are also uncertainties regarding diagnostic thresholds and the way in which results should be interpreted. In this Review, we describe practical strategies for using FISH analysis to detect lymphoma-associated chromosomal abnormalities in routine paraffin-embedded lymphoma biopsies. Furthermore, we provide proposals on how FISH results should be interpreted (including how to calculate cutoff levels for FISH probes), recorded, and reported. An online appendix (available at http://jmd.amjpathol.org) details various simple, yet robust procedures for paraffin FISH analysis; it also provides additional information on the production of FISH probes, evaluating and reporting FISH results, sources for reagents and equipment, and troubleshooting. We hope that these suggestions will make FISH technology for the study of lymphoma biopsies more accessible to routine diagnostic and research laboratories.
在过去十年中，荧光原位杂交（FISH）已成为淋巴瘤诊断和评估中一项牢固确立的技术。然而，该技术在石蜡包埋活检的常规诊断评估中并未广泛应用，最可能的原因是人们认为它在技术上要求更高。关于诊断阈值以及结果的解释方式也存在不确定性。在本综述中，我们描述了在常规石蜡包埋淋巴瘤活检中使用FISH分析检测淋巴瘤相关染色体异常的实用策略。此外，我们就如何解释FISH结果（包括如何计算FISH探针的临界值水平）、记录和报告提出了建议。一个在线附录（可在http://jmd.amjpathol.org获取）详细介绍了石蜡FISH分析的各种简单而可靠的程序；它还提供了关于FISH探针生产、评估和报告FISH结果、试剂和设备来源以及故障排除的更多信息。我们希望这些建议能使用于淋巴瘤活检研究的FISH技术更便于常规诊断和研究实验室使用。
