Manolis Tsatsas, Lee Yi-Chun, Temkin Sarah, Hellman Mira, Nacharaju Vijaya L, Abulafia Ovadia
Department of Obstetrics and Gynecology, The Division of Gynecologic Oncology, State University of New York, Downstate Medical Center, Brooklyn, NY 11203, USA.
Gynecol Obstet Invest. 2006;62(2):103-7. doi: 10.1159/000092856. Epub 2006 Apr 26.
The isoforms of 11beta-hydroxysteroid dehydrogenase (11beta-HSD) types 1 and 2, regulated by ovarian steroids, catalyze the interconversion of glucocorticoids and their 11-keto metabolites. The role of these enzymes in malignancies of human endometrium is unknown. We compare NAD dependent 11beta-HSD (type 2) activity levels among normal human endometrium and endometrial carcinomas of differing grades and histologies.
NAD dependent 11beta-HSD activity was determined in endometrial tissue obtained from patients undergoing hysterectomy for benign or malignant disease (endometroid, serous and carcinosarcomas). Student's t test was utilized with p < 0.05 considered significant. Data are presented as mean +/- SD.
NAD dependent 11beta-HSD activity was present in all endometrial samples. The activities were 0.61+/- 0.27 in normal (n = 9), 0.43 +/- 0.29 in endometrioid endometrial carcinoma (n = 14), 0.50 +/- 0.26 in uterine serous carcinoma (n = 6) and 0.25 +/- 0.37 in carcinosarcomas (n = 9). NAD dependent 11beta-HSD activity was lower in the carcinosarcoma group as compared to normal endometrial tissue (p = 0.03).
NAD dependent type 2 11beta-HSD activity was demonstrated in all normal and endometrial tumors. Enzyme activity in endometroid and uterine serious carcinoma tumors was similar to enzyme activity in normal endometrium. In contrast, carcinosarcomas show significantly lower enzyme activity compared to normal tissue.
11β-羟基类固醇脱氢酶（11β-HSD）1型和2型的同工型受卵巢类固醇调节，催化糖皮质激素与其11-酮代谢产物的相互转化。这些酶在人类子宫内膜恶性肿瘤中的作用尚不清楚。我们比较了正常人子宫内膜以及不同分级和组织学类型的子宫内膜癌中NAD依赖的11β-HSD（2型）活性水平。
测定了因良性或恶性疾病（子宫内膜样癌、浆液性癌和癌肉瘤）接受子宫切除术患者的子宫内膜组织中NAD依赖的11β-HSD活性。采用学生t检验，p<0.05被认为具有统计学意义。数据以平均值±标准差表示。
所有子宫内膜样本中均存在NAD依赖的11β-HSD活性。正常样本（n = 9）中的活性为0.61±0.27，子宫内膜样癌（n = 14）中的活性为0.43±0.29，子宫浆液性癌（n = 6）中的活性为0.50±0.26，癌肉瘤（n = 9）中的活性为0.25±0.37。与正常子宫内膜组织相比，癌肉瘤组中NAD依赖的11β-HSD活性较低（p = 0.03）。
在所有正常子宫内膜和肿瘤中均检测到NAD依赖的2型11β-HSD活性。子宫内膜样癌和子宫浆液性癌肿瘤中的酶活性与正常子宫内膜中的酶活性相似。相比之下，癌肉瘤中的酶活性明显低于正常组织。
