Khozhai L I, Otellin V A
Department of Morphology, Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg.
Neurosci Behav Physiol. 2006 Jun;36(5):513-7. doi: 10.1007/s11055-006-0048-2.
We report here detailed studies of structural changes occurring in the mouse neocortex formed and stratified in conditions of prenatal experimental blockade of serotonin synthesis. Studies were performed using F1(C57BL/CBA) hybrid mice. Endogenous serotonin levels were decreased by exposure of the mice to parachlorophenylalanine, which inhibits the key serotonin synthesis enzyme tryptophan hydroxylase. Offspring brains were studied at 1, 5 and 10 days (n = 10-15 for each time point) of postnatal development. Controls consisted of intact animals at the same periods of development. These experiments showed that prenatal blockade of serotonin synthesis leads to impaired formation of all neocortical layers, impaired growth, development, and differentiation of neurons, and alterations in neuron shape and size. As postnatal development proceeded, significant numbers of neurons died in the brain structures of these animals.
我们在此报告关于在产前实验性阻断血清素合成的条件下形成并分层的小鼠新皮层中发生的结构变化的详细研究。研究使用了F1（C57BL/CBA）杂交小鼠。通过将小鼠暴露于对氯苯丙氨酸来降低内源性血清素水平，对氯苯丙氨酸会抑制关键的血清素合成酶色氨酸羟化酶。在出生后发育的第1、5和10天（每个时间点n = 10 - 15）研究后代的大脑。对照组由处于相同发育阶段的完整动物组成。这些实验表明，产前血清素合成的阻断导致所有新皮层层的形成受损、神经元的生长、发育和分化受损，以及神经元形状和大小的改变。随着出生后发育的进行，这些动物的脑结构中有大量神经元死亡。
