Turell M J, Dohm D J, Fernandez R, Calampa C, O'Guinn M L
Virology Division, U.S. Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, MD 21702-5011, USA.
J Am Mosq Control Assoc. 2006 Mar;22(1):70-5. doi: 10.2987/8756-971X(2006)22[70:VCOPMD]2.0.CO;2.
We evaluated mosquitoes collected in the Amazon Basin, near Iquitos, Peru, for their susceptibility to a subtype IIIC strain of the Venezuelan equine encephalomyelitis complex. This virus had been previously isolated from a pool of mixed Culex vomerifer and Cx. gnomatos captured near Iquitos, Peru, in 1997. After feeding on hamsters with viremias of about 10(8) plaque-forming units of virus per ml, Cx. gnomatos was the most efficient vector. Other species, such as Ochlerotatus fulvus and Psorophora cingulata, although highly susceptible to infection, were not efficient laboratory vectors of this virus due to a significant salivary gland barrier. The Cx. (Culex) species, consisting mostly of Cx. (Cux.) coronator, were nearly refractory to subtype IIIC virus and exhibited both midgut infection as well as salivary gland barriers. Additional studies on biting behavior, mosquito population densities, and vertebrate reservoir hosts of subtype IIIC virus are needed to determine the role that these species play in the maintenance and spread of this virus in the Amazon Basin region.
我们评估了在秘鲁伊基托斯附近的亚马逊盆地采集的蚊子对委内瑞拉马脑炎复合体IIIC亚型毒株的易感性。该病毒先前于1997年从秘鲁伊基托斯附近捕获的一群混合的 vomerifer库蚊和gnomatos库蚊中分离出来。在吸食每毫升含有约10⁸个病毒蚀斑形成单位病毒血症的仓鼠后，gnomatos库蚊是最有效的传播媒介。其他物种，如黄背伊蚊和带纹骚蚊，虽然对感染高度敏感，但由于存在显著的唾液腺屏障，并非该病毒有效的实验室传播媒介。主要由coronator库蚊组成的库蚊属物种对IIIC亚型病毒几乎具有抗性，并表现出中肠感染以及唾液腺屏障。需要对IIIC亚型病毒的叮咬行为、蚊虫种群密度和脊椎动物储存宿主进行更多研究，以确定这些物种在该病毒于亚马逊盆地地区的维持和传播中所起的作用。
