Han Zhao, Lin Guojing, Huang Jianmin, Chi Fanglu, Zhang Lurong
Department of Otorhinolaryngology, Fujian Medical University Affiliated Union Hospital, Fuzhou, China.
Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2006 Feb;20(3):116-8, 121.
To investigate the expressions of extracellular matrix protein-1 (ECM1) in laryngeal carcinoma and their relationships with the growth, metastasis and angiogenesis of tumor.
The immunohistochemical technique was used to detect the expressions of ECM1 and VIII factor in 33 cases of laryngeal tumors. RT-PCR technology was used to test the existence of ECM1 mRNA in both fresh laryngeal tissue and its surrounding tissue (total 5 cases).
ECM1 is expressed in both benign and malignant laryngeal tissues, chiefly in the cellular plasma. But the expression of ECM1 (P<0.01) is significant different between benign and malignant laryngeal tumors. Their expressions are stronger in malignant tissues than in benign ones. In malignant laryngeal tissue, the expression of ECM1 is associated significantly with microvessel density (MVD) (24. 50 +/- 5.96) (Spearman's rho is 0. 505, P <0.05). ECM1 mRNA is found in both laryngeal carcinoma tissue and its surrounding tissue and it seemed no difference.
ECM1 played an important role in the growth, metastasis and angiogenesis of laryngeal carcinoma. The further investigation of ECM1 will benefit the treatment of laryngeal carcinoma and the judgement of its prognosis.
探讨细胞外基质蛋白-1（ECM1）在喉癌中的表达及其与肿瘤生长、转移和血管生成的关系。
采用免疫组化技术检测33例喉肿瘤组织中ECM1和Ⅷ因子的表达。应用逆转录聚合酶链反应（RT-PCR）技术检测5例新鲜喉组织及其周围组织中ECM1 mRNA的存在情况。
ECM1在喉良性和恶性组织中均有表达，主要位于细胞质中。但ECM1在喉良性和恶性肿瘤组织中的表达差异有统计学意义（P<0.01），恶性组织中的表达明显强于良性组织。在喉恶性组织中，ECM1的表达与微血管密度（MVD）（24.50±5.96）显著相关（Spearman等级相关系数为0.505，P<0.05）。在喉癌组织及其周围组织中均检测到ECM1 mRNA，且两者之间无明显差异。
ECM1在喉癌的生长、转移和血管生成中起重要作用。对ECM1的进一步研究将有助于喉癌的治疗及其预后判断。
