Zheng Chuanming, Ge Rongming, Ge Minghua, Sun Xicai
Department of Head and Neck Surgery, Zhejiang Cancer Hospital, Hangzhou, 310022, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2011 Feb;25(3):123-6.
To investigate the expression of chemokine receptor 4 (CXCR4) in laryngeal squamous carcinoma and its correlation with clinicopathology character and the building of microvessel density (MVD). To evaluate its role in the carcinogenesis and progression in laryngeal squamous carcinoma.
The expression of CXCR4 in fresh laryngeal squamous carcinoma tissues and adjacent normal tissues from 42 patients were examined by RT-PCR, immunohistochemical staining and Image-pro-plus software. The numbers of regeneration blood vessels in the laryngeal squamous carcinoma was counted by antibody against factor V associated antigen and immunohistochemical analysis.
The positive expression rate of CXCR4 in tumor samples was significantly higher than that in normal ones (P < 0.01). In tumor samples, the expression of CXCR4 were not associated with age, sex, tumor site and T stage (P > 0.05), while it were higher in tumors of grade III, IV than in grade I, II of pathology classification (P < 0. 01). The expression of CXCR4 were significantly higher in tumors with cervical lymph node metastasis than that in tumor without cervical lymph node metastasis (P < 0.01). The expression of CXCR4 protein and CXCR4 mRNA were at the same level. The expression level of CXCR4 in the laryngeal squamous carcinoma tissue was positively correlated with vascularization.
The higher expression of CXCR4 may play a key role in the invasion and metastasis of laryngeal squamous carcinoma, and were correlated with micro-vascularization.
探讨趋化因子受体4（CXCR4）在喉鳞状细胞癌中的表达情况及其与临床病理特征和微血管密度（MVD）构建的相关性。评估其在喉鳞状细胞癌发生发展中的作用。
采用逆转录-聚合酶链反应（RT-PCR）、免疫组织化学染色及Image-pro-plus软件检测42例喉鳞状细胞癌患者新鲜癌组织及癌旁正常组织中CXCR4的表达。采用抗因子V相关抗原抗体及免疫组织化学分析计数喉鳞状细胞癌中新生血管的数量。
肿瘤样本中CXCR4的阳性表达率显著高于正常样本（P<0.01）。在肿瘤样本中，CXCR4的表达与年龄、性别、肿瘤部位及T分期无关（P>0.05），而在病理分级为III、IV级的肿瘤中高于I、II级（P<0.01）。有颈部淋巴结转移的肿瘤中CXCR4的表达显著高于无颈部淋巴结转移的肿瘤（P<0.01）。CXCR4蛋白和CXCR4 mRNA的表达水平一致。喉鳞状细胞癌组织中CXCR4的表达水平与血管生成呈正相关。
CXCR4的高表达可能在喉鳞状细胞癌的侵袭和转移中起关键作用，并与微血管生成相关。
