Ravelet Corinne, Grosset Catherine, Peyrin Eric
Département de Pharmacochimie Moléculaire UMR 5063 CNRS, Institut de Chimie Moléculaire de Grenoble FR 2607, Université Joseph Fourier, UFR de Pharmacie de Grenoble, Avenue de Verdun, 38240 Meylan, France.
J Chromatogr A. 2006 Jun 2;1117(1):1-10. doi: 10.1016/j.chroma.2006.03.101. Epub 2006 Apr 27.
The development of the systematic evolution of ligands by exponential enrichment (SELEX) process has allowed the isolation of oligonucleotide sequences (referred as aptamers) with the capacity to recognize various classes of target molecules with high affinity and specificity. At the present time, various analytical aptamer-based formats have been developed as suitable tools for the detection and quantification of numerous targets, possessing many potential advantages over the most popular antibody technology. In this review, the aptamer applicability to the separation, purification and quantification of various analytes by liquid chromatography, capillary electrophoresis and electrochromatography is addressed.
指数富集的配体系统进化（SELEX）过程的发展使得能够分离出具有高亲和力和特异性识别各类靶分子能力的寡核苷酸序列（称为适配体）。目前，已经开发出各种基于适配体的分析形式作为检测和定量众多靶标的合适工具，与最流行的抗体技术相比具有许多潜在优势。在这篇综述中，探讨了适配体在液相色谱、毛细管电泳和电色谱中用于各种分析物的分离、纯化和定量的适用性。
