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核酸适配体的分子识别原理及其在液相色谱和毛细管电泳中的应用

Nucleic acid aptamer molecular recognition principles and application in liquid chromatography and capillary electrophoresis.

作者信息

Peyrin Eric

机构信息

Département de Pharmacochimie Moléculaire UMR 5063 CNRS, Institut de Chimie Moléculaire de Grenoble FR 2607, Université Grenoble I (Joseph Fourier), Grenoble, France.

出版信息

J Sep Sci. 2009 May;32(10):1531-6. doi: 10.1002/jssc.200900061.

DOI:10.1002/jssc.200900061
PMID:19370736
Abstract

Nucleic acid aptamers isolated from the systematic evolution of ligands by exponential enrichment (SELEX) have the capacity to recognize various classes of target molecules with high affinity and specificity. In this context, the development of aptamer-based molecular recognition tools has become a very interesting and promising analytical strategy during the last few years. In this review, the molecular recognition features of aptamers as well as the key factors for their practical applicability to the chromatographic and capillary electrophoretic fields are summarized.

摘要

通过指数富集配体系统进化技术（SELEX）分离得到的核酸适体能够以高亲和力和特异性识别各类靶分子。在此背景下，基于适体的分子识别工具的开发在过去几年已成为一种非常有趣且有前景的分析策略。在这篇综述中，总结了适体的分子识别特征以及它们在色谱和毛细管电泳领域实际应用的关键因素。

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