Browne Hyacinth, Taylor Hugh
Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Fertil Steril. 2006 May;85(5):1386-90. doi: 10.1016/j.fertnstert.2005.10.072.
To evaluate HOXA10 expression in endometriosis.
Laboratory study using human tissue.
Academic research center.
PATIENT(S): Eutopic endometrial tissue was collected from 20 fertile women without endometriosis. Ectopic endometrial tissues from pelvic peritoneum, ovary, and lung parenchyma were collected from 20 women undergoing surgery for endometriosis.
INTERVENTION(S): HOXA10 protein expression and localization with immunohistochemistry.
MAIN OUTCOME MEASURE(S): Quantitative analysis of HOXA10 expression, according to H score.
RESULT(S): Both eutopic and ectopic endometrial tissue expressed HOXA10. Ectopic endometrial tissue had lower stromal HOXA10 expression compared with eutopic endometrium. The mean H score for stromal expression of HOXA10 was 7.6 in eutopic endometrium and 1.3 in ectopic endometrial tissue. Glandular epithelium from both eutopic and ectopic endometrium had similarly low HOXA10 expression. HOXA10 was also expressed at low levels in lung parenchyma containing endometriosis and in ovarian endometriomas.
CONCLUSION(S): HOXA10 is expressed in endometriosis at locations outside of the normal HOXA10 expression domain, where it is likely necessary to impart endometrial developmental identity on endometriosis. HOXA10 might be necessary for "de novo" endometrial development. However, the diminished expression of HOXA10 in ectopic endometrium might not allow for normal endometrial development and might contribute to the pathogenesis of endometriosis by creating P resistance.
评估HOXA10在子宫内膜异位症中的表达。
使用人体组织的实验室研究。
学术研究中心。
从20名无子宫内膜异位症的育龄妇女中收集在位子宫内膜组织。从20名接受子宫内膜异位症手术的妇女中收集盆腔腹膜、卵巢和肺实质的异位子宫内膜组织。
采用免疫组织化学法检测HOXA10蛋白表达及定位。
根据H评分对HOXA10表达进行定量分析。
在位和异位子宫内膜组织均表达HOXA10。与在位子宫内膜相比，异位子宫内膜组织中HOXA10的基质表达较低。在位子宫内膜中HOXA10基质表达的平均H评分为7.6，异位子宫内膜组织中为1.3。在位和异位子宫内膜的腺上皮中HOXA10表达均较低。HOXA10在含有子宫内膜异位症的肺实质和卵巢子宫内膜瘤中也低水平表达。
HOXA10在子宫内膜异位症中表达于正常HOXA10表达域之外的部位，在这些部位可能需要赋予子宫内膜异位症子宫内膜发育特征。HOXA10可能是“从头开始”的子宫内膜发育所必需的。然而，HOXA10在异位子宫内膜中的表达降低可能无法实现正常的子宫内膜发育，并可能通过产生孕激素抵抗而促进子宫内膜异位症的发病机制。
