Saroff H A
Laboratory of Biochemical Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Biopolymers. 1991 Aug;31(9):1037-47. doi: 10.1002/bip.360310904.
Clusters of ionizable groups are examined for conditions that develop cooperativity (1) on the binding of protons, and (2) on the binding of an associated ligand when the clusters are shared between domains or subunits in macromolecules. Cooperative binding isotherms for protons have long been observed (but not emphasized as cooperative binding) when studies have been done on clusters for the evaluation of metal ion complexation [A. E. Martell & M. Calvin (1952) Chemistry of the Metal Chelate Compounds, Prentice-Hall, Englewood Cliffs, New Jersey]. Reactions are formulated in this paper to show that anions, chelating to positively charged clusters, are also capable of developing the cooperative binding of protons. Extension of these simple reactions to those where clusters of ionizable groups are shared between domains of macromolecules provides models for cooperative binding, which include the allosteric, Bohr, anion, and cation effects in proteins.
对可电离基团簇进行研究，考察其在以下两种情况下产生协同性的条件：（1）质子结合时；（2）当这些簇在大分子的结构域或亚基之间共享时，相关配体结合时。当对用于评估金属离子络合的簇进行研究时，长期以来都观察到质子的协同结合等温线（但未被强调为协同结合）[A. E. 马特尔和M. 卡尔文（1952年）《金属螯合物的化学》，普伦蒂斯 - 霍尔出版社，新泽西州恩格尔伍德克利夫斯]。本文提出的反应表明，与带正电簇螯合的阴离子也能够产生质子的协同结合。将这些简单反应扩展到可电离基团簇在大分子结构域之间共享的反应，提供了协同结合模型，其中包括蛋白质中的变构效应、玻尔效应、阴离子效应和阳离子效应。
