Ae Park Sun, Choi Myung-Sook, Cho Soo-Yeul, Seo Jung-Sook, Jung Un Ju, Kim Myung-Joo, Sung Mi-Kyung, Park Yong Bok, Lee Mi-Kyung
Department of Food and Nutrition, Yeungnam University, Gyeongsan, 712-749, South Korea.
Life Sci. 2006 Aug 15;79(12):1207-13. doi: 10.1016/j.lfs.2006.03.022. Epub 2006 Mar 27.
This study examines whether anti-diabetic effects of genistein and daidzein are mediated by hepatic glucose and lipid regulating enzyme activities in type 2 diabetic animals. Male C57BL/KsJ-lepr(db)/lepr(db) (db/db) mice and age-matched non-diabetic littermates (db/+) were used in this study. The db/db mice were divided into control, genistein (0.02%, w/w) and daidzein (0.02%, w/w) groups. The blood glucose and HbA(1c) levels were significantly lower in the genistein and daidzein groups than in the control group, while glucose tolerance only was significantly improved in the genistein-supplemented group. The plasma insulin and C-peptide levels did not differ significantly between groups, yet the glucagon level was lower in the genistein and daidzein groups compared to that in the control db/db or db/+ group. The genistein and daidzein supplements increased the insulin/glucagon ratio in the type 2 diabetic animals. While the hepatic glucokinase activity was significantly lower in the db/db control group, the glucose-6-phosphatase and phosphoenolpyruvate carboxykinase activities were significantly higher in the control group compared to the db/+ group. Interestingly, these hepatic glucose metabolizing enzyme activities were reversed by the genistein and daidzein supplementation in db/db mice compared to the control group. The hepatic fatty acid synthase, beta-oxidation and carnitine palmitoyltransferase activities were all significantly lower in the genistein and daidzein groups than in the control group. The genistein and daidzein supplements also improved the plasma total cholesterol, triglyceride, HDL-cholesterol/total cholesterol, free fatty acid and hepatic triglyceride concentrations in the db/db mice. These results suggest that genistein and daidzein exert anti-diabetic effect in type 2 diabetic conditions by enhancing the glucose and lipid metabolism.
本研究考察了染料木黄酮和黄豆苷元的抗糖尿病作用是否通过调节2型糖尿病动物肝脏中葡萄糖和脂质代谢酶的活性来介导。本研究使用了雄性C57BL/KsJ-lepr(db)/lepr(db)（db/db）小鼠以及年龄匹配的非糖尿病同窝小鼠（db/+）。将db/db小鼠分为对照组、染料木黄酮（0.02%，w/w）组和黄豆苷元（0.02%，w/w）组。染料木黄酮组和黄豆苷元组的血糖和糖化血红蛋白（HbA1c）水平显著低于对照组，而仅补充染料木黄酮的组葡萄糖耐量显著改善。各组间血浆胰岛素和C肽水平无显著差异，但染料木黄酮组和黄豆苷元组的胰高血糖素水平低于db/db对照组或db/+对照组。在2型糖尿病动物中，补充染料木黄酮和黄豆苷元可提高胰岛素/胰高血糖素比值。db/db对照组肝脏葡萄糖激酶活性显著降低，与db/+组相比，对照组葡萄糖-6-磷酸酶和磷酸烯醇式丙酮酸羧激酶活性显著升高。有趣的是，与对照组相比，补充染料木黄酮和黄豆苷元可使db/db小鼠的这些肝脏葡萄糖代谢酶活性发生逆转。染料木黄酮组和黄豆苷元组的肝脏脂肪酸合酶、β-氧化和肉碱棕榈酰转移酶活性均显著低于对照组。补充染料木黄酮和黄豆苷元还改善了db/db小鼠的血浆总胆固醇、甘油三酯、高密度脂蛋白胆固醇/总胆固醇、游离脂肪酸和肝脏甘油三酯浓度。这些结果表明，染料木黄酮和黄豆苷元通过增强葡萄糖和脂质代谢在2型糖尿病状态下发挥抗糖尿病作用。
