Fahimi Saeedeh, Oryan Shahrbanoo, Ahmadi Ramesh, Eidi Akram
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Animal Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Iran J Pharm Res. 2023 Feb 1;21(1):e132071. doi: 10.5812/ijpr-132071. eCollection 2022 Dec.
Apoptosis is proposed as a possible mechanism for diabetes-induced hippocampal neuronal cell death. Numerous studies have suggested that the therapeutic properties of plants, such as antioxidant and anti-apoptotic, are effective in improving the complications of diabetes in the hippocampus.
This study aimed to evaluate the anti-apoptotic properties of () in the brain hippocampal cells of diabetic rats.
In this experimental study, 48 male Wistar rats were divided into six groups (n = 8) as follows: Control (C), diabetic (D), harmine (H), diabetic plus harmine (DH), seed extract (S), and diabetic plus seed extract (DS). A single dose of streptozotocin (STZ) (60 mg/kg) was enough to cause diabetes. Seed extract and harmine were given at 150 mg/kg and 6.5 mg/kg, respectively (daily by oral gavage for 28 days). The glucose levels in the blood were measured, and the histological staining of the hippocampus was examined. Percentages of apoptotic hippocampal cells were identified with flow cytometry. and expression was assayed via Real time- polymerase chain reaction (PCR) and Western blot.
In DH (P = 0.001) and DS (P = 0.01) rats, the mean fasting blood glucose level significantly reduced compared with the D group. and expression at both mRNA and protein levels significantly differed between the D group and other groups (P = 0.01). Harmine and the seed extract considerably reduced the Bax/Bcl-2 ratio in the hippocampal cells compared to the D group (P = 0.001).
Streptozotocin-induced apoptosis in the hippocampus of diabetic rats was reduced by administering the seed extract of . The seed extract and its active ingredient, harmine, could be used as anti-apoptotic drugs.
细胞凋亡被认为是糖尿病诱导海马神经元细胞死亡的一种可能机制。大量研究表明，植物的治疗特性，如抗氧化和抗凋亡特性，对改善糖尿病海马并发症有效。
本研究旨在评估（此处原文括号内容缺失）对糖尿病大鼠脑海马细胞的抗凋亡特性。
在本实验研究中，48只雄性Wistar大鼠被分为六组（n = 8），如下：对照组（C）、糖尿病组（D）、骆驼蓬碱组（H）、糖尿病加骆驼蓬碱组（DH）、种子提取物组（S）和糖尿病加种子提取物组（DS）。单剂量链脲佐菌素（STZ）（60 mg/kg）足以诱发糖尿病。种子提取物和骆驼蓬碱分别以150 mg/kg和6.5 mg/kg的剂量给予（每日经口灌胃，持续28天）。测量血糖水平，并检查海马的组织学染色。通过流式细胞术鉴定凋亡海马细胞的百分比。通过实时聚合酶链反应（PCR）和蛋白质印迹法检测（此处原文缺失相关具体物质）和（此处原文缺失相关具体物质）的表达。
与D组相比，DH组（P = 0.001）和DS组（P = 0.01）大鼠的平均空腹血糖水平显著降低。D组与其他组之间，（此处原文缺失相关具体物质）和（此处原文缺失相关具体物质）在mRNA和蛋白质水平的表达存在显著差异（P = 0.01）。与D组相比，骆驼蓬碱和种子提取物显著降低了海马细胞中的Bax/Bcl - 2比值（P = 0.001）。
给予（此处原文缺失相关植物名称）种子提取物可减少链脲佐菌素诱导的糖尿病大鼠海马细胞凋亡。（此处原文缺失相关植物名称）种子提取物及其活性成分骆驼蓬碱可作为抗凋亡药物使用。
