Gessner A, Paulke B R, Müller R H, Göppert T M
Department of Pharmaceutical Technology, Biotechnology and Quality Management, The Free University of Berlin, Berlin, Germany.
Pharmazie. 2006 Apr;61(4):293-7.
Poly (ethylene glycol) (PEG)-grafted nanoparticles have been described as potential intravenously injectable, long-circulating drug carriers. The in vivo behaviour of intravenous administered nanoparticles is decisively influenced by the interaction of the particles with the blood proteins. Two-dimensional electrophoresis (2-DE) was employed to study the protein rejecting properties of PEG-grafted polymer nanoparticles, possessing PEG-200 and PEG-400 chains, respectively. The calculated PEG-chain distances varied between 0.39/0.31 nm (PEG-200) and 0.39/0.34 nm (PEG-400), therefore it was possible to study the influence of high chain densities attained by the use of short PEG chains on the protein adsorption. Apart from a stronger protein rejection of small-MW proteins achieved by PEG-chain distance diminution, the affinity of several proteins for the PEG-chains are shown and discussed. Beside the study of protein adsorption patterns, the total protein mass adsorbed to the particles, as well as the extent of protein desorption prior to 2-DE, was investigated using the bicinchoninic acid (BCA)-protein assay.
聚（乙二醇）（PEG）接枝纳米颗粒已被描述为潜在的可静脉注射的长循环药物载体。静脉给药纳米颗粒的体内行为决定性地受颗粒与血液蛋白质相互作用的影响。采用二维电泳（2-DE）研究分别具有PEG-200和PEG-400链的PEG接枝聚合物纳米颗粒的蛋白质排斥特性。计算得到的PEG链间距在0.39/0.31nm（PEG-200）和0.39/0.34nm（PEG-400）之间变化，因此有可能研究使用短PEG链实现的高链密度对蛋白质吸附的影响。除了通过减小PEG链间距对小分子量蛋白质实现更强的蛋白质排斥外，还展示并讨论了几种蛋白质对PEG链的亲和力。除了研究蛋白质吸附模式外，还使用二辛可宁酸（BCA）蛋白质测定法研究了吸附到颗粒上的总蛋白质质量以及二维电泳之前蛋白质的解吸程度。
