胶体金纳米颗粒与人体血液的相互作用：对颗粒大小的影响及血浆蛋白结合谱分析
Interaction of colloidal gold nanoparticles with human blood: effects on particle size and analysis of plasma protein binding profiles.
作者信息
Dobrovolskaia Marina A, Patri Anil K, Zheng Jiwen, Clogston Jeffrey D, Ayub Nader, Aggarwal Parag, Neun Barry W, Hall Jennifer B, McNeil Scott E
机构信息
Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC-Frederick Inc., NCI-Frederick, Frederick, Maryland 21702, USA.
出版信息
Nanomedicine. 2009 Jun;5(2):106-17. doi: 10.1016/j.nano.2008.08.001. Epub 2008 Dec 13.
Abstract
Nanoparticle size and plasma binding profile contribute to a particle's longevity in the bloodstream, which can have important consequences for therapeutic efficacy. In this study an approximate doubling in nanoparticle hydrodynamic size was observed upon in vitro incubation of 30- and 50-nm colloidal gold in human plasma. Plasma proteins that bind the surface of citrate-stabilized gold colloids have been identified. Effects of protein binding on the nanoparticle hydrodynamic size, elements of coagulation, and the complement system have been investigated. The difference in size measurements obtained from dynamic light scattering, electron microscopy, and scanning probe microscopy are also discussed.
摘要
纳米颗粒的大小和血浆结合特性决定了其在血流中的存留时间，这对治疗效果可能会产生重要影响。在本研究中，观察到30纳米和50纳米的胶体金在人血浆中进行体外孵育后，纳米颗粒的流体动力学尺寸大约增加了一倍。已鉴定出结合柠檬酸盐稳定的金胶体表面的血浆蛋白。研究了蛋白质结合对纳米颗粒流体动力学尺寸、凝血因子和补体系统的影响。还讨论了通过动态光散射、电子显微镜和扫描探针显微镜获得的尺寸测量结果的差异。
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