Atemnkeng Magnus A, Plaizier-Vercammen Jacqueline, Schuermans Annette
Vrije Universiteit Brussel, Pharmaceutical Institute, Pharmaceutical Technology and Physical Pharmacy, Laarbeeklaan 103, 1090 Brussels, Belgium.
Int J Pharm. 2006 Jul 24;317(2):161-6. doi: 10.1016/j.ijpharm.2006.03.013. Epub 2006 Mar 22.
Equilibrium dialysis on povidone-iodine-solutions (Braunol, standardized Betadine and non-standardized iso-Betadine reveal that the amount of available iodine, free iodine, iodide and triiodide varies significantly both in the undiluted and diluted forms. These differences are reflected in the different bactericidal activity against Staphyloccus aureus as determined by the standard quantitative in vitro suspension test. The amount of available iodine is not an appropriate measure for an assessment of the microbicidal activity. For this, the free iodine has to be determined by means of equilibrium dialysis. The free iodine concentration in the Braunol concentrate was found to be 22 mg/L, in the standardized Betadine 9.7 mg/L and in the non-standardized Betadine concentrate only 2.1mg/L. Because of the atypical behaviour of iodophores and the increase of free iodine at dilution and because of a bactericidal level of free iodine of 5mg/L, Braunol and standardized Betadine can be employed as disinfectant as such, iso-Betadine has to be diluted before use. Summarizing all results, it can be stated that Braunol is superior to standardized Betadine and unstandardized iso-Betadine both as to the release of free iodine in the undiluted and in the diluted forms as in the killing rate of S. aureus.
对聚维酮碘溶液（Braunol、标准的碘伏和非标准的异碘伏）进行平衡透析发现，无论是未稀释还是稀释形式，有效碘、游离碘、碘化物和三碘化物的含量都有显著差异。这些差异在通过标准定量体外悬液试验测定的对金黄色葡萄球菌的不同杀菌活性中得到体现。有效碘的含量并非评估杀菌活性的合适指标。为此，必须通过平衡透析来测定游离碘。发现Braunol浓缩液中的游离碘浓度为22mg/L，标准碘伏中为9.7mg/L，非标准碘伏浓缩液中仅为2.1mg/L。由于碘伏的非典型行为以及稀释时游离碘的增加，且游离碘的杀菌水平为5mg/L，Braunol和标准碘伏本身可作为消毒剂使用，异碘伏在使用前必须稀释。总结所有结果可以表明，无论是在未稀释和稀释形式下游离碘的释放量还是在对金黄色葡萄球菌的杀灭率方面，Braunol都优于标准碘伏和非标准的异碘伏。
