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表面消毒剂杀菌活性的评估。IV. AOAC使用稀释法和凯尔西-赛克斯试验。

The assessment of the bactericidal activity of surface disinfectants. IV. The AOAC use-dilution method and the Kelsey-Sykes test.

作者信息

Reybrouck G

机构信息

Public Health Leuven, School of Public Health, University of Leuven, Belgium.

出版信息

Zentralbl Hyg Umweltmed. 1992 Feb;192(5):432-7.

PMID:1554401
Abstract

The AOAC use-dilution method was applied in order to assess the bactericidal activity of 29 commercial preparations for surface disinfection and the Kelsey-Sykes test under clean conditions for 38 preparations. The results of both tests are compared with those of the in vitro test (with the disinfectant diluted in distilled water, in water of standardized hardness, and in a 0.2% albumin solution), those of the European suspension test under clean and under dirty conditions, and those of four practical tests (the AFNOR test, the DGHM test, the QCT and the QSDT). Both the AOAC use-dilution method and the Kelsey-Sykes test under clean conditions are more severe than the suspension tests and, in the case of Pseudomonas aeruginosa, even than most practical tests. The results of both tests correlate well to one another, and to the results of in vitro test, but less to those of the European suspension test. For the practical tests the correlation depends on the testing technique and on the test organism.

摘要

采用AOAC使用稀释法评估29种市售表面消毒制剂的杀菌活性,并在清洁条件下对38种制剂进行凯尔西-赛克斯试验。将这两种试验的结果与体外试验(消毒剂分别用蒸馏水、标准硬度水和0.2%白蛋白溶液稀释)的结果、清洁和脏污条件下的欧洲悬液试验结果以及四项实际试验(法国标准化协会试验、德国卫生与微生物学会试验、QCT和QSDT)的结果进行比较。AOAC使用稀释法和清洁条件下的凯尔西-赛克斯试验都比悬液试验更严格,对于铜绿假单胞菌,甚至比大多数实际试验更严格。这两种试验的结果相互之间以及与体外试验的结果相关性良好,但与欧洲悬液试验的结果相关性较小。对于实际试验,相关性取决于测试技术和受试微生物。

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