Caspar-Bauguil S, Cousin B, André M, Nibbelink M, Galinier A, Periquet B, Casteilla L, Pénicaud L
UMR 5018 CNRS-UPS, IFR 31, CHU Rangueil, TSA 50032, 31059 Toulouse Cedex 9, France.
Exp Cell Res. 2006 Jul 15;312(12):2195-202. doi: 10.1016/j.yexcr.2006.03.023. Epub 2006 May 2.
Ancestral lymphoid cells reside in adipose tissues, and their numbers are highly altered in obesity. Leptin, production of which is correlated to fat mass, is strongly involved in the relationships between adipose tissues and immune system. We investigated in epididymal (EPI) and inguinal (ING) fat pads to determine whether 1) lymphocyte phenotypes were correlated to the tissue weight and 2) leptin was involved in such relationships. Immunohistological analyses revealed a tight relationship between the T and NK lymphocytes of the stromal vascular fraction and adipocytes. We identified a significant negative and positive correlation between EPI weight and the percentage of NK and total T cells respectively by cytofluorometric analyses. The NK and ancestral gammadelta T cell contents were directly dependent of leptin since they increased significantly in high-fat (HF) diet mice but not in leptin-deficient (ob/ob) mice as compared to control. By contrast, the alphabeta T cell content seemed independent of leptin because their percentages increased significantly with the EPI weight whatever the type of mice (control, HF, ob/ob). The present study suggests that adipose tissues present, according to their localization, different immunological mechanisms that might be involved in the regulation of adipose cells functions and proliferations.
原始淋巴细胞存在于脂肪组织中，其数量在肥胖状态下会发生显著改变。瘦素的产生与脂肪量相关，在脂肪组织与免疫系统的关系中起着重要作用。我们对附睾（EPI）和腹股沟（ING）脂肪垫进行了研究，以确定：1）淋巴细胞表型是否与组织重量相关；2）瘦素是否参与了这种关系。免疫组织学分析揭示了基质血管部分的T淋巴细胞和NK淋巴细胞与脂肪细胞之间存在紧密关系。通过细胞荧光分析，我们分别确定了附睾重量与NK细胞百分比以及总T细胞百分比之间存在显著的负相关和正相关。NK细胞和原始γδT细胞的含量直接依赖于瘦素，因为与对照组相比，高脂（HF）饮食小鼠的这些细胞含量显著增加，而瘦素缺乏（ob/ob）小鼠则没有。相反，αβT细胞的含量似乎与瘦素无关，因为无论小鼠类型（对照、HF、ob/ob）如何，其百分比都随附睾重量的增加而显著增加。本研究表明，根据脂肪组织的位置，其存在不同的免疫机制，这些机制可能参与脂肪细胞功能和增殖的调节。
