自发性癫痫大鼠毒蕈碱型胆碱能、γ-氨基丁酸（A）和苯环利定受体的特征；体外定量放射自显影分析

Characteristics of muscarinic cholinergic, gamma-aminobutyric acid(A) and phencyclidine receptors in spontaneously epileptic rats; in vitro quantitative autoradiographic analysis.

作者信息

Murase K, Nabeshima T, Kameyama T, Sasa M, Takaori S, Ujihara H, Ishihara K, Yamada J, Serikawa T

机构信息

Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Meijo University, Nagoya, Japan.

出版信息

Neurosci Lett. 1991 Sep 30;131(1):1-4. doi: 10.1016/0304-3940(91)90323-l.

DOI:10.1016/0304-3940(91)90323-l

PMID:1665211

Abstract

Characteristics of muscarinic cholinergic (mACh), gamma-aminobutyric acid(A) (GABAA) and phencyclidine (PCP) receptors in the spontaneously epileptic rats (SER), which exhibit both absence-like seizures and tonic convulsion, were examined using in vitro quantitative autoradiography. Computer analysis using autoradiographic technique revealed that the amount of the specific binding of [3H]quinuclidinyl benzilate (QNB) to mACh receptors in the striatum of SER was more than that of zitter rats, not exhibiting both seizures and convulsion. However, the specific bindings of [3H]muscimol and [3H]N-(1-[2-thienyl]cyclohexyl)3,4-piperidine (TCP) to GABAA and PCP receptors, respectively, of SER were not different from those of zitter rats in various regions tested. These results suggest that hyperfunction of mACh receptors in the striatum is involved in the appearance of absence-like seizures and tonic convulsion of SER.

摘要

利用体外定量放射自显影技术，检测了既出现失神样发作又有强直性惊厥的自发性癫痫大鼠（SER）中，毒蕈碱型胆碱能（mACh）、γ-氨基丁酸A（GABAA）和苯环利定（PCP）受体的特征。采用放射自显影技术进行计算机分析显示，SER纹状体中[3H]喹核醇基苯甲酸酯（QNB）与mACh受体的特异性结合量，高于既不出现癫痫发作也无惊厥的颤抖大鼠。然而，在各测试区域中，SER的[3H]蝇蕈醇和[3H]N-（1-[2-噻吩基]环己基）3,4-哌啶（TCP）分别与GABAA和PCP受体的特异性结合，与颤抖大鼠并无差异。这些结果表明，纹状体中mACh受体功能亢进与SER失神样发作和强直性惊厥的出现有关。